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DIAPH3 Antibody (PA5-115123) in ICC/IF
Immunocytochemistry analysis of DIAPH3 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115123) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
产品信息
PA5-115123
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human DIAPH3(Accession Q9NSV4), corresponding to amino acid residues T825-S875.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2899759
产品详细信息
Antibody detects endogenous levels of total DIAPH3.
靶标信息
This gene encodes a member of the diaphanous subfamily of the formin family. Members of this family are involved in actin remodeling and regulate cell movement and adhesion. Mutations in this gene are associated with autosomal dominant auditory neuropathy 1. Multiple transcript variants encoding different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: diaphanous homolog 3; diaphanous-related formin; diaphanous-related formin 3; Diaphanous-related formin-3; DKFZp434C0931; DKFZp686A13178; DRF3; FLJ34705; MDia2; p134mDIA2; Protein diaphanous homolog 3
基因别名: 4930417P13Rik; AN; AUNA1; DIA2; DIAP3; DIAPH3; DRF3; mDia2; mKIAA4117; NSDAN; p134MDia2
UniProt ID: (Human) Q9NSV4, (Mouse) Q9Z207, (Rat) F1LVW7
Entrez Gene ID: (Human) 81624, (Mouse) 56419, (Rat) 290396
