Invitrogen

DIS3 Polyclonal Antibody

DIS3 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 8

DIS3 Antibody (PA5-58723) in ICC/IF

Immunofluorescent staining of DIS3 in human cell line U-2 OS using a DIS3 Polyclonal Antibody (Product # PA5-58723) shows localization to nucleus and cytosol.

DIS3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

DIS3 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. U-87 mg ATCC were transfected with, control siRNA, DIS3 siRNA (probe #1), and DIS3 siRNA (probe #2) and a decrease in signal intensity was observed in Western Blot application using anti-DIS3 Polyclonal Antibody (Product # PA5-58723) used at a dilution of 0.4 µg/mL. Anti-GAPDH was used as a loading control. {KD}

产品信息

PA5-58723

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

0.25-2 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human DIS3. Recombinant protein control fragment (Product #RP-97922).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2640613

产品详细信息

Immunogen sequence: EAYILFVRKN AIVVLIPKYG LEGTVFFEEK DKPNPQLIYD DEIPSLKIED TVFHVFDKVK VKIMLDSSNL QHQKIRMSLV EPQIPGISIP TDTSN

Highest antigen sequence identity to the following orthologs: Mouse - 86%, Rat - 87%.

靶标信息

The exosome is involved in a multitude of cellular RNA processing and degradation events. DIS3, also known as exosome complex exonuclease RRP44, is a ribonuclease that acts directly in the processing, turnover, and surveillance of a large number of distinct RNA species. DIS3 localizes to both the cytoplasm and the nucleus and contains one PINc domain. It is widely expressed with highest expression in testis and is required for processing of 7S pre-RNA into a mature nuclear complex and, ultimately, for proper mitotic progression. Abnormal expression levels of DIS3 may be associated with colon cancer, suggesting a role for DIS3 in tumorigenesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: bA555G22.1; DIS3 exosome endoribonuclease and 3'-5' exoribonuclease; DIS3 mitotic control homolog; Exosome complex exonuclease RRP44; exosome component 11; mitotic control protein dis3 homolog; Protein DIS3 homolog; Ribosomal RNA-processing protein 44; RP11-342J4.3

查看更多收起

基因别名: 2810028N01Rik; DIS3; dis3p; EXOSC11; KIAA1008; RRP44

查看更多收起

UniProt ID: (Human) Q9Y2L1

查看更多收起

Entrez Gene ID: (Human) 22894

查看更多收起

功能

3'-5'-exoribonuclease activity RNA binding endonuclease activity guanyl-nucleotide exchange factor activity protein binding exoribonuclease

参与通路

rRNA processing mRNA catabolic process rRNA catabolic process regulation of mRNA stability positive regulation of GTPase activity exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay CUT catabolic process RNA phosphodiester bond hydrolysis, exonucleolytic