Invitrogen

DLL4 (delta-like 4) Monoclonal Antibody (HMD4-1), Functional Grade, eBioscience™

产品信息

16-5948-82

应用

建议稀释比

已发表文章

功能检测 (Functional)

Assay-Dependent

产品规格

种属反应

Mouse, Rat

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

HMD4-1

偶联物

Functional Grade Functional Grade Functional Grade

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

no preservative

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_11043100

产品详细信息

Description: This HMD4-1 monoclonal antibody reacts with the extracellular domain of mouse Delta-like 4, one of five type I transmembrane proteins that serves as a Notch receptor ligand. Upon binding the Notch receptor (e.g., Notch 1-4), Delta-like 4 undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase. The second cleavage event releases the Notch intracellular domain (NICD), which subsequently translocates into the nucleus, heterodimerizes with the DNA-binding protein RBP-J, recruits co-activator molecules, and ultimately activates transcription. Delta-like 4 is expressed by thymic lymphoid and stromal cells, as well as macrophages and dendritic cells in the spleen. This molecule is also expressed in non-immune cells, including vascular smooth muscle and endothelial cells. Delta-like 4 plays a role in T cell development, regulation of experimental autoimmune encephalomyelitis, and the formation of mature vasculature.

The HMD4-1 monoclonal antibody has been reported to have blocking activity and crossreact to rat.

Applications Reported: The HMD4-1 antibody has been reported for use in functional assays.

Applications Tested: This HMD4-1 antibody has been tested by flow cytometric analysis of mouse Delta-like 4-transfected cells. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

靶标信息

Delta-like ligand 4 (DLL4), also know as Delta-4, is a homologue of the Drosophila delta protein, and functions as a transmembrane bound ligand to the notch receptor Notch1. DLL4 is expressed in the vasculature and plays a critical role in vascular development. It is induced by vascular endothelial growth factor (VEGF), as a negative feedback regulator to regulate angiogenic sprouting and promote the formulation of a differentiated vascular network. DLL4 has been found to be strongly expressed in tumor vessels of clear-cell renal tumors and bladder cancer, and inhibition of DLL4 results in increased vascular proliferation but defective maturation. This in turn leads to a decrease in tumor growth, with no apparent toxicity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。