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DMPK Antibody (PA5-99271) in ICC/IF
Immunocytochemistry analysis of DMPK in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with DMPK polyclonal antibody (Product # PA5-99271) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-99271
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human DMPK(Accession Q09013), corresponding to amino acid residues L20-E40.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2818204
产品详细信息
Antibody detects endogenous levels of total DMPK.
靶标信息
DMPK is a serine/threonine kinase expressed in various muscle and nerve tissues. Mutations in the untranslated region of the DMPK gene are a characteristic of myotonic dystrophy. The protein encoded by this gene is a serine-threonine kinase that is closely related to other kinases that interact with members of the Rho family of small GTPases. Substrates for this enzyme include myogenin, the beta-subunit of the L-type calcium channels, and phospholemman. The 3' untranslated region of this gene contains 5-37 copies of a CTG trinucleotide repeat. Expansion of this unstable motif to 50-5,000 copies causes myotonic dystrophy type I, which increases in severity with increasing repeat element copy number. Repeat expansion is associated with condensation of local chromatin structure that disrupts the expression of genes in this region.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DM protein kinase; DM-kinase; DM1 protein kinase; DMK; DMPK; dystrophia myotonica-protein kinase; MT-PK; myotonic dystrophy associated protein kinase; Myotonic dystrophy protein kinase; myotonin protein kinase A; Myotonin-protein kinase; thymopoietin homolog
基因别名: DM; DM1; DM1PK; DMK; DMPK; MDPK; MT-PK
UniProt ID: (Human) Q09013
Entrez Gene ID: (Human) 1760
regulation of sodium ion transport
protein phosphorylation
cellular calcium ion homeostasis
nuclear envelope organization
regulation of heart contraction
muscle cell apoptotic process
regulation of myotube differentiation
regulation of skeletal muscle contraction by calcium ion signaling
regulation of excitatory postsynaptic membrane potential involved in skeletal muscle contraction
peptidyl-serine phosphorylation
regulation of catalytic activity
regulation of synapse structural plasticity
regulation of cardiac conduction
