Invitrogen

DMRTA2 Polyclonal Antibody

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2 篇参考文献

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抗体检测数据 (1)
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DMRTA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DMRTA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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DMRTA2 Antibody (PA5-60237) in IHC (P)

Immunohistochemical analysis of DMRTA2 in human colon using DMRTA2 Polyclonal Antibody (Product # PA5-60237) shows moderate cytoplasmic positivity in glandular cells.

DMRTA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DMRTA2 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

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DMRTA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of DMRTA2 in human colon using DMRTA2 Polyclonal Antibody (Product # PA5-60237) shows moderate cytoplasmic positivity in glandular cells.

DMRTA2 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Fig. 4 A DMRTA2 methylation in SV-HUC-1 and various BC cell lines detected by MSP. MSP products in lanes U and M indicate the presence of unmethylated and methylated DMRTA2 , respectively. B Quantification of m DMRTA2 in SV-HUC-1 and various BC cell lines by qMSP with ACTB as the reference gene. Data is shown as mean +- s.d. of three independent experiments ( n = 3). C Level of DMRTA2 mRNA expression in SV-HUC-1 and various BC cell lines by RT-qPCR with GAPDH as the reference gene. Data is shown as mean +- s.d. of three independent experiments ( n = 3). (D) Elevation of DMRTA2 mRNA expression in SV-HUC-1 and various BC cell lines after 5'-Aza-dC treatment (demethylation) by RT-qPCR with GAPDH as the reference gene. Data is shown as mean +- s.d. of three independent experiments ( n = 3). E and F , Level of DMRTA2 protein expression detected in SV-HUC-1 and various BC cell lines by western blot analysis with GAPDH as the internal control. Data is shown as mean +- s.d. of three independent experiments ( n = 3). G and H , Effect of 5'-Aza-dC treatment (demethylation) on DMRTA2 expression in the same set of cell lines by western blot analysis. Data shown as mean +- s.d. of three independent experiments ( n = 3). I and J , Examination of DMRTA2 expression in BC and adjacent normal tissues by IHC. Left panel and right panel show negative and positive staining for two distinct tissue sections. K and L , Morphological features of tumor and adjacent normal tissue sections revealed by H

产品信息

PA5-60237

应用

建议稀释比

已发表文章

免疫印迹 (WB)

-

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免疫组化 (IHC)

-

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免疫组化（石蜡） (IHC (P))

1:20-1:50

-

产品规格

种属反应

Human

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human DMRTA2. Recombinant protein control fragment (Product #RP-98462).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.05 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2640636

产品详细信息

Immunogen sequence: GSEAKLQKFD LFPKTLLQAG RPGSPLPPPV KPLSPDGADS GPGTSSPEVR PGSGSENGDG ESF

Highest antigen sequence identity to the following orthologs: Mouse - 92%, Rat - 94%.

靶标信息

DMRTA2 may be involved in sexual development.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A novel and sensitive DNA methylation marker for the urine-based liquid biopsies to detect bladder cancer.

BMC cancer

Deng L,Chao H,Deng H,Yu Z,Zhao R,Huang L,Gong Y,Zhu Y,Wang Q,Li F,Liu L,He L,Tang Z,Liao C,Qi Y,Wang X,Zeng T,Zou H

Published figure using DMRTA2 polyclonal antibody (Product # PA5-60237) in Immunohistochemistry

Fri May 06 00:00:00 UTC 2022

A novel and sensitive DNA methylation marker for the urine-based liquid biopsies to detect bladder cancer.

BMC cancer

Deng L,Chao H,Deng H,Yu Z,Zhao R,Huang L,Gong Y,Zhu Y,Wang Q,Li F,Liu L,He L,Tang Z,Liao C,Qi Y,Wang X,Zeng T,Zou H

Published figure using DMRTA2 polyclonal antibody (Product # PA5-60237) in Immunohistochemistry

Fri May 06 00:00:00 UTC 2022

生物信息学

蛋白别名: DMRT-like family A2; Doublesex- and mab-3-related transcription factor 5; Doublesex- and mab-3-related transcription factor A2

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基因别名: DMRT5; DMRTA2

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UniProt ID: (Human) Q96SC8

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Entrez Gene ID: (Human) 63950

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core promoter proximal region sequence-specific DNA binding transcription factor activity, sequence-specific DNA binding protein homodimerization activity metal ion binding protein heterodimerization activity DNA-binding transcription factor

positive regulation of neuroblast proliferation regulation of transcription, DNA-templated brain development sex differentiation cerebral cortex regionalization skeletal muscle cell differentiation neuron fate specification dopaminergic neuron differentiation