Invitrogen

DNA Ligase III Polyclonal Antibody

DNA Ligase III Antibody in Immunocytochemistry (ICC/IF)

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DNA Ligase III Antibody (PA5-21480) in ICC/IF

DNA Ligase III Polyclonal Antibody detects LIG3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LIG3 protein stained by DNA Ligase III Polyclonal Antibody (Product # PA5-21480) diluted at 1:500. Blue: Hoechst 33342 staining.

DNA Ligase III Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DNA Ligase III Antibody in Western Blot (WB)

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产品信息

PA5-21480

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

体外检测 (IV)

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产品规格

种属反应

Human

已发表种属

Chicken

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 116 and 433 of DNA ligase 3 (Uniprot ID#P49916)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.81 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_11153448

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2.

Predicted reactivity: Mouse (92%), Rat (91%), Pig (94%), Rhesus Monkey (97%), Bovine (91%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

There are four distinct ligase proteins found in mammalian cells. The activity of these DNA ligases is required for the joining of DNA strand breaks that occur during DNA replication, repair and recombination. DNA Ligase III gene product migrates as a 100 kDa (a-form) protein in somatic tissues, is ubiquitously expressed and complexes with XRCC-1 and a 97 kDa (beta form) protein that is found in testis. DNA Ligase III is involved in rejoining DNA single-strand breaks and in the base excision repair pathway.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

RAD52 Adjusts Repair of Single-Strand Breaks via Reducing DNA-Damage-Promoted XRCC1/LIG3α Co-localization.

Cell reports

Wang J,Oh YT,Li Z,Dou J,Tang S,Wang X,Wang H,Takeda S,Wang Y

PA5-21480 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot, In Vitro Assay to demonstrate that blocking RAD52 oligomerization that disrupts RAD52's DSBR, while retaining its ssDNA binding capacity that is required for RAD52's inhibitory effects on SSBR, sensitizes cells to different DNA-damaging agents.