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DNA2 Antibody (PA5-115131) in ICC/IF
Immunocytochemistry analysis of DNA2 in HepG2 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115131). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
Product Details
PA5-115131
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human DNA2(Accession P51530), corresponding to amino acid residues T430-G480.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2899767
Product Specific Information
Antibody detects endogenous levels of total DNA2L.
Target Information
DNA2 is a conserved helicase/nuclease involved in themaintenance of mitochondrial and nuclear DNA stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA replication ATP-dependent helicase-like homolog; DNA replication ATP-dependent helicase/nuclease DNA2; DNA replication helicase 2 homolog; DNA2-like helicase; hDNA2
Gene Aliases: DNA2; DNA2L; E130315B21Rik; hDNA2; KIAA0083
UniProt ID: (Human) P51530, (Mouse) Q6ZQJ5
Entrez Gene ID: (Human) 1763, (Mouse) 327762
DNA binding
DNA helicase activity
helicase activity
nuclease activity
protein binding
ATP binding
ATPase activity
site-specific endodeoxyribonuclease activity, specific for altered base
5'-flap endonuclease activity
5'-3' DNA helicase activity
single-stranded DNA-dependent ATPase activity
metal ion binding
4 iron, 4 sulfur cluster binding
nucleotide binding
catalytic activity
endonuclease activity
hydrolase activity
phosphoric ester hydrolase activity
T/G mismatch-specific endonuclease activity
retroviral 3' processing activity
iron-sulfur cluster binding
DNA replication checkpoint
DNA double-strand break processing
DNA replication
mitochondrial DNA replication
DNA repair
base-excision repair
cellular response to DNA damage stimulus
metabolic process
DNA duplex unwinding
DNA replication, Okazaki fragment processing
DNA replication, removal of RNA primer
mitochondrial DNA repair
positive regulation of DNA replication
nucleic acid phosphodiester bond hydrolysis
telomere maintenance via recombination
telomere maintenance
DNA synthesis involved in DNA repair
strand displacement
regulation of signal transduction by p53 class mediator
mitotic telomere maintenance via semi-conservative replication
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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