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DNMT1 Antibody (PA1-880) in ICC/IF
Immunofluorescence analysis of DNMT1 was performed using 70% confluent log phase K-562 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with DNMT1 Polyclonal Antibody (Product # PA1-880) at 2 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000... View More
产品信息
PA1-880
产品规格
种属反应
Human,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues A(171) K G P A K R K P Q E E S E(185) of human Dnmt1.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_325912
产品详细信息
PA1-880 detects DNA methyltransferase 1 (Dmnt1) from HeLa cell nuclear extracts.
PA1-880 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~180 kDa protein representing Dnmt1 from HeLa cell nuclear extract. A doublet between 40 and 45 kDa is also seen on Western blots using this antibody.
The PA1-880 immunogen is a synthetic peptide corresponding to residues A(171) K G P A K R K P Q E E S E(185) of human Dnmt1. PA1-880 immunizing peptide (Cat. # PEP-136) is available for use in neutralization and control experiments.
靶标信息
This gene encodes a subunit of the augmin complex, which regulates centrosome and mitotic spindle integrity, and is necessary for the completion of cytokinesis. The encoded protein was identified by interaction with ubiquitin C-terminal hydrolase 37. Alternative splicing results in multiple transcript variants.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CXXC-type zinc finger protein 9; DNA (cytosine-5)-methyltransferase 1; DNA methyltransferase (cytosine-5) 1; DNA methyltransferase HsaI; DNA methyltransferase I; DNA MTase HsaI; DNA MTase RnoIP; Dnmt1; FLJ16293; m.RnoIP; MCMT; MGC104992
基因别名: ADCADN; AIM; CXXC9; DNMT; DNMT1; HSN1E; m.HsaI; MCMT
UniProt ID: (Human) P26358
Entrez Gene ID: (Human) 1786, (Rat) 84350
DNA binding
double-stranded DNA binding
RNA binding
DNA (cytosine-5-)-methyltransferase activity
protein binding
zinc ion binding
methyl-CpG binding
DNA-methyltransferase activity
protein domain specific binding
estrogen receptor binding
histone deacetylase binding
unmethylated CpG binding
DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates
promoter-specific chromatin binding
chromatin binding
transcription factor binding
methyltransferase activity
S-adenosylmethionine-dependent methyltransferase activity
negative regulation of transcription from RNA polymerase II promoter
DNA methylation
transcription, DNA-templated
regulation of transcription, DNA-templated
brain development
aging
response to heat
response to toxic substance
response to organic substance
response to ionizing radiation
maintenance of DNA methylation
response to lead ion
DNA methylation on cytosine within a CG sequence
regulation of gene expression
positive regulation of gene expression
response to activity
gene silencing
chromatin modification
neuron differentiation
response to caffeine
response to nutrient levels
response to estradiol
response to lipopolysaccharide
DNA methylation on cytosine
response to vitamin A
response to testosterone
cellular response to platelet-derived growth factor stimulus
response to antidepressant
regulation of cell proliferation
response to drug
DNA hypermethylation
response to ethanol
negative regulation of transcription, DNA-templated
S-adenosylhomocysteine metabolic process
S-adenosylmethioninamine metabolic process
S-adenosylmethionine metabolic process
positive regulation of histone H3-K4 methylation
negative regulation of histone H3-K9 methylation
cellular response to amino acid stimulus
cellular response to lead ion
cellular response to transforming growth factor beta stimulus
C-5 methylation of cytosine
cellular response to nerve growth factor stimulus
Ras protein signal transduction
covalent chromatin modification
negative regulation of gene expression, epigenetic
positive regulation of methylation-dependent chromatin silencing
