Invitrogen

DNMT1 Monoclonal Antibody (60B1220.1)

DNMT1 Antibody in Immunocytochemistry (ICC/IF)

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DNMT1 Antibody (MA5-16169) in ICC/IF

Immunofluorescence analysis of DNMT1 was performed using 70% confluent log phase K-562 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with DNMT1 Monoclonal Antibody (60B1220.1) (Product # MA5-16169) at 1:10 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766... View More

DNMT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DNMT1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

DNMT1 Antibody — Antibody specificity was demonstrated by detection of enrichment of the target protein at specific gene loci. Chromatin Immunoprecipitation (ChIP) was performed using Anti-DNMT1 monoclonal antibody (Product # MA5-16169) using PCR primer pairs over mir-137, POMC, IL1R2 (positive) and SAT2 satellite repeats (negative). {RE}

产品信息

MA5-16169

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:500

免疫组化（冰冻） (IHC (F))

1:500

免疫组织化学（悬浮细胞） (IHC (Free))

1:500

免疫细胞化学 (ICC/IF)

1:10

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

1 - 5 µg/mL

免疫沉淀 (IP)

1:10-1:500

查看1篇已发表文章 1篇已发表文章

染色质免疫沉淀实验 (ChIP)

1:20-1:1,000

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产品规格

种属反应

Bovine, Human, Mouse, Sheep, Pig, Rat

已发表种属

Gerbil, Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

60B1220.1

抗原

Synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human Dnmt1.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2537688

产品详细信息

Suggested positive control: human kidney (IHC), mouse ES cells (WB).

靶标信息

This gene encodes a subunit of the augmin complex, which regulates centrosome and mitotic spindle integrity, and is necessary for the completion of cytokinesis. The encoded protein was identified by interaction with ubiquitin C-terminal hydrolase 37. Alternative splicing results in multiple transcript variants.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

DNMT1-Mediated DNA Methylation Targets CDKN2B to Promote the Repair of Retinal Ganglion Cells in Streptozotocin-Induced Mongolian Gerbils during Diabetic Retinopathy.

Computational and mathematical methods in medicine

Wang X,Zhang J,Liao Y,Jin Y,Yu X,Li H,Yang Q,Li X,Chen R,Wu D,Zhu H

MA5-16169 was used in Western Blot to explore the role of DNA cytosine-5-methyltransferase 1 (DNMT1) in the development of early diabetic retinopathy and its potential underlying mechanism.

Mon Mar 28 00:00:00 UTC 2022

DNMT1-Mediated DNA Methylation Targets CDKN2B to Promote the Repair of Retinal Ganglion Cells in Streptozotocin-Induced Mongolian Gerbils during Diabetic Retinopathy.

Computational and mathematical methods in medicine

Wang X,Zhang J,Liao Y,Jin Y,Yu X,Li H,Yang Q,Li X,Chen R,Wu D,Zhu H

MA5-16169 was used in Western Blot to explore the role of DNA cytosine-5-methyltransferase 1 (DNMT1) in the development of early diabetic retinopathy and its potential underlying mechanism.

Mon Mar 28 00:00:00 UTC 2022

LncRNA affects epigenetic reprogramming of porcine embryo development by regulating global epigenetic modification and the downstream gene SIN3A.

Frontiers in physiology

Zhang D,Zhou Y,Huang R,Zhai Y,Wu D,An X,Zhang S,Shi L,Li Q,Kong X,Yu H,Li Z

Published figure using DNMT1 monoclonal antibody (Product # MA5-16169) in Immunocytochemistry

Tue Oct 04 00:00:00 UTC 2022

Myocardial tissue-specific Dnmt1 knockout in rats protects against pathological injury induced by Adriamycin.

Laboratory investigation; a journal of technical methods and pathology

Wu TT,Ma YW,Zhang X,Dong W,Gao S,Wang JZ,Zhang LF,Lu D

MA5-16169 was used in Western Blot, Immunocytochemistry to investigate the impact of genome-wide cardiac DNA methyltransferase deficiency on the physiology and pathological processes of heart tissue under stress within an in vivo rat model.

Wed Jul 01 00:00:00 UTC 2020

Myocardial tissue-specific Dnmt1 knockout in rats protects against pathological injury induced by Adriamycin.

Laboratory investigation; a journal of technical methods and pathology

Wu TT,Ma YW,Zhang X,Dong W,Gao S,Wang JZ,Zhang LF,Lu D

Wed Jul 01 00:00:00 UTC 2020

DNMT1 and HDAC2 Cooperate to Facilitate Aberrant Promoter Methylation in Inorganic Phosphate-Induced Endothelial-Mesenchymal Transition.

PloS one

Tan X,Xu X,Zeisberg M,Zeisberg EM

MA5-16169 was used in western blot to study the interaction between DNMT1 and HDAC2 and how they cooperate to facilitate aberrant promoter methylation in inorganic phosphate-induced endothelial-mesenchymal transition

Tue Jul 26 00:00:00 UTC 2016

DNMT1 and HDAC2 Cooperate to Facilitate Aberrant Promoter Methylation in Inorganic Phosphate-Induced Endothelial-Mesenchymal Transition.

PloS one

Tan X,Xu X,Zeisberg M,Zeisberg EM

Tue Jul 26 00:00:00 UTC 2016

DNMT1 and HDAC2 Cooperate to Facilitate Aberrant Promoter Methylation in Inorganic Phosphate-Induced Endothelial-Mesenchymal Transition.

PloS one

Tan X,Xu X,Zeisberg M,Zeisberg EM

Tue Jul 26 00:00:00 UTC 2016

生物信息学

蛋白别名: CXXC-type zinc finger protein 9; DNA (cytosine 5 ) methyltransferase 1; DNA (cytosine-5)-methyltransferase 1; DNA methyltransferase (cytosine 5 ) methyltransferase; DNA methyltransferase (cytosine-5) 1; DNA methyltransferase b; DNA methyltransferase HsaI; DNA methyltransferase I; DNA methyltransferase MmuI; DNA MTase HsaI; DNA MTase MmuI; DNA MTase RnoIP; Dnmt1; FLJ16293; m.RnoIP; MCMT; MGC104992

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基因别名: ADCADN; AIM; CXXC9; DNMT; DNMT1; Dnmt1o; HSN1E; m.HsaI; m.MmuI; MCMT; Met-1; Met1; MommeD2; MTase; Uim

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UniProt ID: (Bovine) Q24K09, (Human) P26358, (Mouse) P13864

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功能

DNA binding DNA (cytosine-5-)-methyltransferase activity zinc ion binding promoter-specific chromatin binding double-stranded DNA binding RNA binding protein binding methyl-CpG binding DNA-methyltransferase activity protein domain specific binding estrogen receptor binding histone deacetylase binding unmethylated CpG binding DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates chromatin binding transcription factor binding methyltransferase activity S-adenosylmethionine-dependent methyltransferase activity catalytic activity transferase activity metal ion binding DNA metabolism protein DNA methyltransferase nucleic acid metabolism protein

参与通路

chromatin organization Ras protein signal transduction maintenance of DNA methylation C-5 methylation of cytosine positive regulation of methylation-dependent chromatin silencing negative regulation of transcription from RNA polymerase II promoter DNA methylation transcription, DNA-templated brain development aging response to heat response to toxic substance response to ionizing radiation DNA methylation on cytosine within a CG sequence positive regulation of gene expression response to activity gene silencing covalent chromatin modification neuron differentiation response to caffeine response to estradiol response to lipopolysaccharide response to vitamin A response to testosterone cellular response to platelet-derived growth factor stimulus response to antidepressant regulation of cell proliferation DNA hypermethylation response to ethanol negative regulation of gene expression, epigenetic S-adenosylhomocysteine metabolic process S-adenosylmethioninamine metabolic process S-adenosylmethionine metabolic process positive regulation of histone H3-K4 methylation negative regulation of histone H3-K9 methylation cellular response to amino acid stimulus cellular response to lead ion cellular response to transforming growth factor beta stimulus cellular response to nerve growth factor stimulus regulation of transcription, DNA-templated response to organic substance response to lead ion regulation of gene expression chromatin modification response to nutrient levels DNA methylation on cytosine response to drug negative regulation of transcription, DNA-templated metabolic process methylation

DNMT1 Monoclonal Antibody (60B1220.1)

DNMT1 Antibody (MA5-16169) in ICC/IF

产品信息

MA5-16169

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

免疫组化（冰冻） (IHC (F))

免疫组织化学（悬浮细胞） (IHC (Free))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

免疫沉淀 (IP)

染色质免疫沉淀实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

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DNMT1 Monoclonal Antibody (60B1220.1)

DNMT1 Antibody (MA5-16169) in ICC/IF

产品信息

MA5-16169

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

免疫组化（冰冻） (IHC (F))

免疫组织化学（悬浮细胞） (IHC (Free))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

免疫沉淀 (IP)

染色质免疫沉淀 实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)