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DNMT3L Antibody (PA5-76619) in WB
Western blot was performed using Anti-DNMT3L Polyclonal Antibody (Product # PA5-76619) and a 43 kDa band corresponding to DNMT3L was observed across the cell lines and tissues tested. Uncharacterized bands of ~ 110 kDa and ~30 kDa were observed in tissues. Modified whole cell extracts (1% SDS) (30 µg lysate) of BeWo (Lane 1), HeLa (Lane 2), Jurkat (Lane 3), Caco-2 (Lane 4), PC-3 (Lane 5), SK-BR-3 (Lane 6), tissue extracts (30 µg lysate) of Mouse Testis (Lane 7), Rat Testis (Lane 8), Mouse Kidney (Lane 9) and Mouse Liver (Lane 10) were elec... View More
产品信息
PA5-76619
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant full length Human DNMT3L.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.2, with 50% glycerol
内含物
0.02% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2720346
产品详细信息
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
靶标信息
Methylation of DNA at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting, and development. Three families of mammalian DNA methyltransferases have been identified: DNMT1, DNMT2, and DNMT3. DNMT1 is constitutively expressed in proliferating cells and functions as a maintenance methyltransferase, transferring proper methylation patterns to newly synthesized DNA during replication. DNMT3A and DNMT3B are strongly expressed in embryonic stem cells with reduced expression in adult somatic tissues. DNMT3A and DNMT3B function as de novo methyltransferases that methylate previously unmethylated regions of DNA. DNMT2 is expressed at low levels in adult somatic tissues and its inactivation affects neither de novo nor maintenance DNA methylation. DNMT3L is a catalytically inactive regulatory factor for the DNMT3A and DNMT3B de novo methyltransferases that is expressed at low levels in embryonic stem cells, testis, ovaries, and thymus. These de novo methyltransferases consist of a heterotetrameric complex containing two molecules of DNMT3L, and either two molecules of DNMT3A or DNMT3B. DNMT3L contains an amino-terminal ATRX-DNMT3-DNMT3L (ADD) domain and a carboxy-terminal methyltransferase-like domain.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: cytosine-5-methyltransferase 3-like protein; DNA (cytosine-5)-methyltransferase 3-like; DNA (cytosine-5-)-methyltransferase 3-like; Dnmt 3L; human cytosine-5-methyltransferase 3-like protein; MGC1090
基因别名: D6Ertd14e; DNMT3L; ecat7
UniProt ID: (Human) Q9UJW3, (Mouse) Q9CWR8
Entrez Gene ID: (Human) 29947, (Mouse) 54427
DNA methylation
regulation of gene expression by genetic imprinting
spermatogenesis
positive regulation of catalytic activity
negative regulation of transcription, DNA-templated
in utero embryonic development
placenta development
methylation
chorionic trophoblast cell differentiation
genetic imprinting
