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DNMT3L Antibody (PA5-89316) in WB
Western blot analysis of DNMT3L in extracts of various cell lines. Samples were incubated with DNMT3L Polyclonal antibody (Product # PA5-89316) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
产品信息
PA5-89316
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 151-386 of human DNMT3L (NP_7870631)
偶联物
形式
Liquid
浓度
1.06 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2805488
产品详细信息
Immunogen sequence: SGLLQRRRKW RSQLKAFYDR ESENPLEMFE TVPVWRRQPV RVLSLFEDIK KELTSLGFLE SGSDPGQLKH VVDVTDTVRK DVEEWGPFDL VYGATPPLGH TCDRPPSWYL FQFHRLLQYA RPKPGSPRPF FWMFVDNLVL NKEDLDVASR FLEMEPVTIP DVHGGSLQNA VRVWSNIPAI RSRHWALVSE EELSLLAQNK QSSKLAAKWP TKLVKNCFLP LREYFKYFST ELTSSL; Positive Samples: HeLa, Jurkat, 293T, Mouse testis, Mouse thymus, Mouse ovary, Rat testis; Cellular Location: Nucleus
靶标信息
Methylation of DNA at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting, and development. Three families of mammalian DNA methyltransferases have been identified: DNMT1, DNMT2, and DNMT3. DNMT1 is constitutively expressed in proliferating cells and functions as a maintenance methyltransferase, transferring proper methylation patterns to newly synthesized DNA during replication. DNMT3A and DNMT3B are strongly expressed in embryonic stem cells with reduced expression in adult somatic tissues. DNMT3A and DNMT3B function as de novo methyltransferases that methylate previously unmethylated regions of DNA. DNMT2 is expressed at low levels in adult somatic tissues and its inactivation affects neither de novo nor maintenance DNA methylation. DNMT3L is a catalytically inactive regulatory factor for the DNMT3A and DNMT3B de novo methyltransferases that is expressed at low levels in embryonic stem cells, testis, ovaries, and thymus. These de novo methyltransferases consist of a heterotetrameric complex containing two molecules of DNMT3L, and either two molecules of DNMT3A or DNMT3B. DNMT3L contains an amino-terminal ATRX-DNMT3-DNMT3L (ADD) domain and a carboxy-terminal methyltransferase-like domain.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: cytosine-5-methyltransferase 3-like protein; DNA (cytosine-5)-methyltransferase 3-like; DNA (cytosine-5-)-methyltransferase 3-like; Dnmt 3L; human cytosine-5-methyltransferase 3-like protein; MGC1090
基因别名: D6Ertd14e; DNMT3L; ecat7
UniProt ID: (Human) Q9UJW3, (Mouse) Q9CWR8
Entrez Gene ID: (Human) 29947, (Mouse) 54427
DNA methylation
regulation of gene expression by genetic imprinting
spermatogenesis
positive regulation of catalytic activity
negative regulation of transcription, DNA-templated
in utero embryonic development
placenta development
methylation
chorionic trophoblast cell differentiation
genetic imprinting
