Invitrogen

DOCK4 Polyclonal Antibody

抗体检测数据 (3)

DOCK4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DOCK4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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DOCK4 Antibody (PA5-143947) in IHC (P)

Immunohistochemistry analysis of DOCK4 in paraffin-embedded section of human skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. Samples were incubated with DOCK4 Polyclonal antibody (Product # PA5-143947) using a dilution of 2 μg/mL overnight at 4°C. Peroxidase Conjugated Goat Anti-rab... View More

DOCK4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DOCK4 Antibody in Western Blot (WB)

DOCK4 Antibody in Flow Cytometry (Flow)

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DOCK4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of DOCK4 in paraffin-embedded section of human skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. Samples were incubated with DOCK4 Polyclonal antibody (Product # PA5-143947) using a dilution of 2 μg/mL overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

DOCK4 Antibody in Western Blot (WB) — Western blot analysis of DOCK4 using DOCK4 antibody (Product # PA5-143947). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human 293T whole cell lysates; Lane 2: rat L6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DOCK4 antigen affinity purified polyclonal antibody (Product # PA5-143947) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5,000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DOCK4 at approximately 250 kDa. The expected band size for DOCK4 is at 225 kDa.

DOCK4 Antibody in Flow Cytometry (Flow) — Flow cytometry of DOCK4 in SiHa cells (Blue line). The cells were blocked with 10% normal goat serum, and then incubated with DOCK4 polyclonal antibody (Product # PA5-143947) (1 µg/1x10^6 cells) for 30 min at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Unlabeled sample (Red line) was also used as a control. DyLight®488 conjugated goat anti-rabbit IgG (5-10 μg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C.

产品信息

PA5-143947

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.25-0.5 µg/mL

-

免疫组化（石蜡） (IHC (P))

2-5 µg/mL

-

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

-

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

-

产品规格

种属反应

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human DOCK4 recombinant protein (Position: K191-I1837).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_3075161

产品详细信息

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

靶标信息

SRC2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: dedicator of cytokinesis 4; Dedicator of cytokinesis protein 4

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基因别名: DOCK4; KIAA0716; RGD1561724

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UniProt ID: (Human) Q8N1I0

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Entrez Gene ID: (Human) 9732, (Rat) 366608

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guanyl-nucleotide exchange factor activity GTPase activator activity protein binding SH3 domain binding PDZ domain binding receptor tyrosine kinase binding Rac GTPase binding guanyl-nucleotide exchange factor

参与通路

small GTPase mediated signal transduction positive regulation of GTPase activity cell chemotaxis negative regulation of vascular smooth muscle contraction positive regulation of vascular associated smooth muscle cell migration