Invitrogen

DRD1 Polyclonal Antibody

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抗体检测数据 (2)

DRD1 Antibody in Immunohistochemistry (IHC)

DRD1 Antibody in Immunohistochemistry (IHC)

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DRD1 Antibody (720358) in IHC

Sections of human cerebral cortex were dewaxed, microwaved in citric acid and incubated with Anti-Dopamine Receptor D1 Rabbit Polyclonal Antibody (Product # 720358 at a concentration of 2 µg/mL). Sections were then sequentially treated with biotinylated anti-rabbit IgG and AB solution. Sections were then developed in AEC and lightly counterstained with hematoxylin. Inset, for adsorption controls the antibody was incubated with 10 µg/mL of the peptide used for immunizations (+ Peptide). Note, staining of neuronal somata and dendrites is com... View More

DRD1 Antibody in Immunohistochemistry (IHC)

DRD1 Antibody in Western Blot (WB)

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DRD1 Antibody in Immunohistochemistry (IHC) — Sections of human cerebral cortex were dewaxed, microwaved in citric acid and incubated with Anti-Dopamine Receptor D1 Rabbit Polyclonal Antibody (Product # 720358 at a concentration of 2 µg/mL). Sections were then sequentially treated with biotinylated anti-rabbit IgG and AB solution. Sections were then developed in AEC and lightly counterstained with hematoxylin. Inset, for adsorption controls the antibody was incubated with 10 µg/mL of the peptide used for immunizations (+ Peptide). Note, staining of neuronal somata and dendrites is completely neutralized by preincubation of the antibody with its immunizing peptide.

DRD1 Antibody in Western Blot (WB) — HEK 293 cells were transiently transfected with Dopamine Receptor D1 or Dopamine Receptor D5. After 48 h, cells were lysed in detergent buffer and glycoproteins were enriched using wheat germ lectin agarose beads. Receptors were eluted from the beads using SDS sample buffer for 20 min at 45 °C. Extracts were separated on 7.5% SDS-polyacrylamide gels and blotted onto PVDF membranes. Membranes were then incubated with rabbit polyclonal Anti-Dopamine Receptor D1 Rabbit Polyclonal Antibody (Product # 720358 at a concentration of 2 µg/mL). Bands from 70-100 kDa corresponding to Dopamine receptor D1 was observed.

产品信息

720358

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

-

免疫组化 (IHC)

2 µg/mL

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Peptide corresponding to Human DRD1 (aa 427-446)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.1% BSA, 30% glycerol

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2633266

产品详细信息

These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.

This antibody is predicted to react with Monkey, Cat and Bovine.

靶标信息

The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: D(1A) dopamine receptor; D1a receptor; Dopamine 1 receptor; Dopamine D1 receptor

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基因别名: DADR; DRD1; DRD1A

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UniProt ID: (Human) P21728

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Entrez Gene ID: (Human) 1812

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dopamine neurotransmitter receptor activity, coupled via Gs G-protein alpha-subunit binding dopamine neurotransmitter receptor activity protein binding drug binding protein phosphatase binding angiotensin receptor binding D3 dopamine receptor binding protein complex binding dopamine binding protein heterodimerization activity ATPase binding G-protein coupled receptor

temperature homeostasis conditioned taste aversion behavioral fear response synaptic transmission, dopaminergic response to amphetamine negative regulation of protein kinase activity protein import into nucleus G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger adenylate cyclase-activating G-protein coupled receptor signaling pathway activation of adenylate cyclase activity adenylate cyclase-activating dopamine receptor signaling pathway synapse assembly learning memory mating behavior grooming behavior adult walking behavior visual learning positive regulation of adenylate cyclase activity involved in G-protein coupled receptor signaling pathway astrocyte development response to activity dopamine transport transmission of nerve impulse neuronal action potential regulation of vasoconstriction calcium-mediated signaling dentate gyrus development striatum development orbitofrontal cortex development cerebral cortex GABAergic interneuron migration positive regulation of cell migration negative regulation of cell migration peristalsis positive regulation of cAMP biosynthetic process response to food response to estradiol response to retinoic acid cellular response to insulin stimulus response to nicotine operant conditioning social behavior response to antidepressant regulation of dopamine metabolic process vasodilation negative regulation of circadian sleep/wake cycle, sleep dopamine metabolic process response to drug maternal behavior response to amino acid positive regulation of potassium ion transport response to morphine response to ethanol positive regulation of membrane potential glucose import habituation sensitization behavioral response to cocaine regulation of long-term neuronal synaptic plasticity response to steroid hormone positive regulation of release of sequestered calcium ion into cytosol positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway regulation of dopamine uptake involved in synaptic transmission positive regulation of synaptic transmission, glutamatergic prepulse inhibition phospholipase C-activating dopamine receptor signaling pathway long-term synaptic potentiation long term synaptic depression cellular response to hypoxia cellular response to catecholamine stimulus positive regulation of long-term synaptic potentiation positive regulation of feeding behavior