Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
DRD3 Antibody (PA5-89944) in WB
Western blot analysis of DRD3 in extracts of various cell lines. Samples were incubated with DRD3 Polyclonal antibody (Product # PA5-89944) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-89944
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Dopamine Receptor D3 (Dopamine Receptor D3 (DRD3)) (NP_0007872)
Conjugate
Form
Liquid
Concentration
0.65 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805858
Product Specific Information
Immunogen sequence: MASLSQLSGH LNYTCGAENS TGASQARPHA YYALSYCALI LAIVFGNGLV CMAVLKERAL QTTTNYLVVS LAVADLLVAT LVMPWVVYLE VTGGVWNFSR; Positive Samples: Mouse brain, Rat brain; Cellular Location: Cell membrane, Multi-pass membrane protein
Target Information
This gene encodes the D3 subtype of the five (D1-D5) dopamine receptors. The activity of the D3 subtype receptor is mediated by G proteins which inhibit adenylyl cyclase. This receptor is localized to the limbic areas of the brain, which are associated with cognitive, emotional, and endocrine functions. Genetic variation in this gene may be associated with susceptibility to hereditary essential tremor 1. Alternative splicing of this gene results in transcript variants encoding different isoforms, although some variants may be subject to nonsense-mediated decay (NMD).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: D(3) dopamine receptor; D3 receptor; D3nf; Dopamine 3 receptor; Dopamine D3 receptor; dopamine receptor 3; dopaminergic receptor D3
Gene Aliases: D3R; Drd3
UniProt ID: (Rat) P19020, (Mouse) P30728
Entrez Gene ID: (Rat) 29238, (Mouse) 13490
dopamine neurotransmitter receptor activity, coupled via Gi/Go
dopamine neurotransmitter receptor activity
protein binding
drug binding
protein domain specific binding
D1 dopamine receptor binding
dopamine binding
signal transducer activity
G-protein coupled receptor activity
G-protein coupled amine receptor activity
G-protein coupled receptor
negative regulation of transcription from RNA polymerase II promoter
synaptic transmission, dopaminergic
response to amphetamine
regulation of blood volume by renin-angiotensin
G-protein coupled receptor internalization
cellular calcium ion homeostasis
G-protein coupled receptor signaling pathway
adenylate cyclase-activating dopamine receptor signaling pathway
negative regulation of adenylate cyclase activity
adenylate cyclase-inhibiting dopamine receptor signaling pathway
dopamine receptor signaling pathway
chemical synaptic transmission
locomotory behavior
positive regulation of cell proliferation
visual learning
regulation of dopamine secretion
regulation of lipid metabolic process
regulation of cAMP metabolic process
negative regulation of sodium:proton antiporter activity
positive regulation of cytokinesis
circadian regulation of gene expression
response to histamine
gastric emptying
positive regulation of renal sodium excretion
regulation of locomotion
regulation of multicellular organism growth
response to cocaine
response to drug
response to morphine
regulation of circadian sleep/wake cycle, sleep
response to ethanol
negative regulation of blood pressure
positive regulation of mitotic nuclear division
positive regulation of transcription from RNA polymerase II promoter
acid secretion
behavioral response to cocaine
negative regulation of oligodendrocyte differentiation
arachidonic acid secretion
negative regulation of protein secretion
musculoskeletal movement, spinal reflex action
negative regulation of protein kinase B signaling
prepulse inhibition
negative regulation of dopamine receptor signaling pathway
positive regulation of dopamine receptor signaling pathway
regulation of locomotion involved in locomotory behavior
signal transduction
sensory perception of chemical stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support