Invitrogen

DYKDDDDK Tag Recombinant Rabbit Monoclonal Antibody (8H8L17)

DYKDDDDK Tag Antibody in Immunocytochemistry (ICC/IF)

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DYKDDDDK Tag Antibody (701629) in ICC/IF

Immunofluorescent analysis of DYKDDDDK (C terminal) was done on 70% confluent DYKDDDDK (C terminal) transfected with HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with DYKDDDDK (C terminal) Recombinant Rabbit Monoclonal Antibody (Produc... View More

DYKDDDDK Tag Antibody in Western Blot (WB)

DYKDDDDK Tag Antibody in Immunoprecipitation (IP)

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产品信息

701629

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

查看6篇已发表文章 6篇已发表文章

免疫细胞化学 (ICC/IF)

1 - 5 µg/mL

查看2篇已发表文章 2篇已发表文章

免疫沉淀 (IP)

2.5 µg/mL

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Tag

已发表种属

Tag

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

8H8L17

抗原

Synthetic peptide (DYKDDDDK)

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2532497

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Identification of Novel Variants of Thyroid Hormone Receptor Interaction Protein 13 That Cause Female Infertility Characterized by Zygotic Cleavage Failure.

Frontiers in physiology

Hu H,Zhang S,Guo J,Meng F,Chen X,Gong F,Lu G,Zheng W,Lin G

701629 was used in Immunocytochemistry to highlight a critical role of TRIP13 in meiosis and mitosis, as well as expand the genetic and phenotypic spectra of TR1P13 variants with respect to female infertility, especially in relation to ZCF.

Sat Jul 16 00:00:00 UTC 2022

The S-palmitoylome and DHHC-PAT interactome of Drosophila melanogaster S2R+ cells indicate a high degree of conservation to mammalian palmitoylomes.

PloS one

Porcellato E,González-Sánchez JC,Ahlmann-Eltze C,Elsakka MA,Shapira I,Fritsch J,Navarro JA,Anders S,Russell RB,Wieland FT,Metzendorf C

701629 was used in Western Blotting to present the palmitoylome of Drosophila S2R+ cells, comprising 198 proteins, an estimated 3.5% of expressed genes in these cells.

Tue Aug 16 00:00:00 UTC 2022

Selenoprotein T Protects Endothelial Cells against Lipopolysaccharide-Induced Activation and Apoptosis.

Antioxidants (Basel, Switzerland)

Merk D,Ptok J,Jakobs P,von Ameln F,Greulich J,Kluge P,Semperowitsch K,Eckermann O,Schaal H,Ale-Agha N,Altschmied J,Haendeler J

701629 was used in Immunocytochemistry-immunofluorescence to propose that Selenoprotein T (SELENOT) as well as APEX1(1-20) can protect endothelial cells (EC) against activation and apoptosis and could provide new therapeutic approaches in the treatment of sepsis.

Tue Sep 07 00:00:00 UTC 2021

Foot-and-Mouth Disease Virus 3B Protein Interacts with Pattern Recognition Receptor RIG-I to Block RIG-I-Mediated Immune Signaling and Inhibit Host Antiviral Response.

Journal of immunology (Baltimore, Md. : 1950)

Zhang X,Zhu Z,Wang C,Yang F,Cao W,Li P,Du X,Zhao F,Liu X,Zheng H

701629 was used in Western Blot to demonstrate the significant role of 3B protein in suppression of type I IFN production and host antiviral response in both human embryonic kidney HEK293T cells and porcine kidney PK-15 cells.

Thu Oct 15 00:00:00 UTC 2020

Porcine DNAJB6 promotes PCV2 replication via enhancing the formation of autophagy in host cells.

Veterinary research

Han C,Du Q,Zhu L,Chen N,Luo L,Chen Q,Yin J,Wu X,Tong D,Huang Y

701629 was used in Immunoprecipitation to show a novel function of pDNAJB6 in regulation of porcine circovirus replication that pDNAJB6 enhances the formation of autophagy to promote viral replication through interacting with viral capsid protein during PCV2 infection.

Thu May 07 00:00:00 UTC 2020

The NALCN channel complex is voltage sensitive and directly modulated by extracellular calcium.

Science advances

Chua HC,Wulf M,Weidling C,Rasmussen LP,Pless SA

701629 was used in Western Blotting to show that robust function of NALCN in heterologous systems requires co-expression of UNC79, UNC80, and FAM155A.

Wed Apr 01 00:00:00 UTC 2020

Cellular p32 Is a Critical Regulator of Porcine Circovirus Type 2 Nuclear Egress.

Journal of virology

Wang T,Du Q,Niu Y,Zhang X,Wang Z,Wu X,Yang X,Zhao X,Liu SL,Tong D,Huang Y

701629 was used in Western Blotting to demonstrate p32 as a critical regulator of PCV2 nuclear egress.

Sun Dec 01 00:00:00 UTC 2019

A contractile injection system stimulates tubeworm metamorphosis by translocating a proteinaceous effector.

eLife

Ericson CF,Eisenstein F,Medeiros JM,Malter KE,Cavalcanti GS,Zeller RW,Newman DK,Pilhofer M,Shikuma NJ

Published figure using DYKDDDDK Tag recombinant monoclonal antibody (Product # 701629) in Western Blot

Tue Sep 17 00:00:00 UTC 2019

Porcine MKRN1 Modulates the Replication and Pathogenesis of Porcine Circovirus Type 2 by Inducing Capsid Protein Ubiquitination and Degradation.

Journal of virology

Wang T,Du Q,Wu X,Niu Y,Guan L,Wang Z,Zhao X,Liu SL,Tong D,Huang Y

701629 was used in Western Blotting to characterise a putative porcine Makorin RING finger protein 1 variant, an N-terminal-truncated variant of putative full-size porcine MKRN1 which has a unique expression pattern resulting from the porcine mkrn1 gene and which interacts with PCV2 Cap.

Fri Jun 01 00:00:00 UTC 2018

生物信息学

蛋白别名: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag

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