Invitrogen

DYKDDDDK Tag Monoclonal Antibody (L5), Alexa Fluor™ 488

DYKDDDDK Tag Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 10

DYKDDDDK Tag Antibody (MA1-142-A488) in ICC/IF

Immunofluorescent analysis of HeLa cells transfected with a construct containing a FLAG® Epitope Tag. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 inPBS for 15 minutes at room temperature and blocked with 3% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were stained with AlexaFluor 488 conjugated FLAG-tag monoclonal antibody (Product # MA1-142-A48... View More

DYKDDDDK Tag Antibody in Western Blot (WB)

DYKDDDDK Tag Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

DYKDDDDK Tag Antibody in Immunohistochemistry (IHC)

Product Image Gallery

产品信息

MA1-142-A488

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组织化学（多聚甲醛固定） (IHC (PFA))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:25-1:100

查看7篇已发表文章 7篇已发表文章

流式细胞分析 (Flow)

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Tag

已发表种属

Tag

宿主/亚型

Rat / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

抗原

DYKDDDDK-tagged mouse Langerin

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

4° C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

AB_2610653

产品详细信息

similar epitope as the FLAG tag from Sigma

靶标信息

DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

GAPDH mediates plant reovirus-induced incomplete autophagy for persistent viral infection in leafhopper vector.

Autophagy

Chen Q,Zhang Y,Yang H,Wang X,Ren J,Jia D,Chen H,Wei T

MA1-142-A488 was used in Immunocytochemistry to reveal a positive regulation of immune response in insect vectors during viral infection.

Sat Apr 01 00:00:00 UTC 2023

NaV1.6 dysregulation within myocardial T-tubules by D96V calmodulin enhances proarrhythmic sodium and calcium mishandling.

The Journal of clinical investigation

Tarasov M,Struckman HL,Olgar Y,Miller A,Demirtas M,Bogdanov V,Terentyeva R,Soltisz AM,Meng X,Min D,Sakuta G,Dunlap I,Duran AD,Foster MP,Davis JP,Terentyev D,Györke S,Veeraraghavan R,Radwański PB

MA1-142-A488 was used in Immunohistochemistry (PFA fixed) to demonstrate that D96V-CaM promotes arrhythmias by dysregulating LTCC and NaV1.6 within T-tubules and thereby, facilitating aberrant Ca2+ release.

Mon Apr 03 00:00:00 UTC 2023

An integrated model for Gpr124 function in Wnt7a/b signaling among vertebrates.

Cell reports

America M,Bostaille N,Eubelen M,Martin M,Stainier DYR,Vanhollebeke B

MA1-142-A488 was used in Immunocytochemistry, Western Blot, Immunoprecipitation to provide insights into the evolution of Gpr124/Reck function across the vertebrate clade, a receptor complex uniquely implicated in Wnt ligand-specific cellular responses.

Tue May 31 00:00:00 UTC 2022

Rapid generation of potent antibodies by autonomous hypermutation in yeast.

Nature chemical biology

Wellner A,McMahon C,Gilman MSA,Clements JR,Clark S,Nguyen KM,Ho MH,Hu VJ,Shin JE,Feldman J,Hauser BM,Caradonna TM,Wingler LM,Schmidt AG,Marks DS,Abraham J,Kruse AC,Liu CC

MA1-142-A488 was used in Flow Cytometry to develop the autonomous hypermutation yeast surface display technique for recombinant antibody generation.

Fri Oct 01 00:00:00 UTC 2021

High-purity production and precise editing of DNA base editing ribonucleoproteins.

Science advances

Jang HK,Jo DH,Lee SN,Cho CS,Jeong YK,Jung Y,Yu J,Kim JH,Woo JS,Bae S

MA1-142-A488 was used in Immunohistochemistry to applied NG PAM-targetable ABE RNPs to in vivo gene correction in retinal degeneration 12 (rd12) model mice.

Sun Aug 01 00:00:00 UTC 2021

M448R and MGF505-7R: Two African Swine Fever Virus Antigens Commonly Recognized by ASFV-Specific T-Cells and with Protective Potential.

Vaccines

Bosch-Camós L,López E,Collado J,Navas MJ,Blanco-Fuertes M,Pina-Pedrero S,Accensi F,Salas ML,Mundt E,Nikolin V,Rodríguez F

MA1-142-A488 was used in Immunocytochemistry-immunofluorescence to use data from the SLAI-peptide repertoire presented by a single set of african swine fever virus-infected porcine alveolar macrophages to generate a complex DNA vaccine composed by 15 plasmids encoding the individual peptide-bearing ORFs.

Fri May 14 00:00:00 UTC 2021

Oxidative pentose phosphate pathway and glucose anaplerosis support maintenance of mitochondrial NADPH pool under mitochondrial oxidative stress.

Bioengineering & translational medicine

Moon SJ,Dong W,Stephanopoulos GN,Sikes HD

Published figure using DYKDDDDK Tag monoclonal antibody (Product # MA1-142-A488) in Immunocytochemistry

Tue Sep 01 00:00:00 UTC 2020

Structural and functional characterization of G protein-coupled receptors with deep mutational scanning.

eLife

Jones EM,Lubock NB,Venkatakrishnan AJ,Wang J,Tseng AM,Paggi JM,Latorraca NR,Cancilla D,Satyadi M,Davis JE,Babu MM,Dror RO,Kosuri S

MA1-142-A488 was used in Immunocytochemistry to characterize large libraries of GPCR variants in human cell lines with a barcoded transcriptional reporter of G protein signal transduction.

Wed Oct 21 00:00:00 UTC 2020

Short Stature is Progressive in Patients with Heterozygous NPR2 Mutations.

The Journal of clinical endocrinology and metabolism

Hanley PC,Kanwar HS,Martineau C,Levine MA

MA1-142-A488 was used in Immunocytochemistry to show that iallelic NPR2 mutations cause severe skeletal dysplasia (AMDM), whereas heterozygous mutations lead to a subtler phenotype characterized by progressive short stature with by increasing loss of height potential with age.

Thu Oct 01 00:00:00 UTC 2020

In vivo stabilization of OPA1 in hepatocytes potentiates mitochondrial respiration and gluconeogenesis in a prohibitin-dependent way.

The Journal of biological chemistry

Li L,Martin-Levilain J,Jiménez-Sánchez C,Karaca M,Foti M,Martinou JC,Maechler P

Published figure using DYKDDDDK Tag monoclonal antibody (Product # MA1-142-A488) in Immunofluorescence

Fri Aug 23 00:00:00 UTC 2019

In vivo stabilization of OPA1 in hepatocytes potentiates mitochondrial respiration and gluconeogenesis in a prohibitin-dependent way.

The Journal of biological chemistry

Li L,Martin-Levilain J,Jiménez-Sánchez C,Karaca M,Foti M,Martinou JC,Maechler P

Published figure using DYKDDDDK Tag monoclonal antibody (Product # MA1-142-A488) in Immunofluorescence

Fri Aug 23 00:00:00 UTC 2019

In vivo stabilization of OPA1 in hepatocytes potentiates mitochondrial respiration and gluconeogenesis in a prohibitin-dependent way.

The Journal of biological chemistry

Li L,Martin-Levilain J,Jiménez-Sánchez C,Karaca M,Foti M,Martinou JC,Maechler P

Published figure using DYKDDDDK Tag monoclonal antibody (Product # MA1-142-A488) in Immunofluorescence

Fri Aug 23 00:00:00 UTC 2019

Raptor-Mediated Proteasomal Degradation of Deamidated 4E-BP2 Regulates Postnatal Neuronal Translation and NF-κB Activity.

Cell reports

Kouloulia S,Hallin EI,Simbriger K,Amorim IS,Lach G,Amvrosiadis T,Chalkiadaki K,Kampaite A,Truong VT,Hooshmandi M,Jafarnejad SM,Skehel P,Kursula P,Khoutorsky A,Gkogkas CG

Published figure using DYKDDDDK Tag monoclonal antibody (Product # MA1-142-A488) in Immunocytochemistry

Tue Dec 10 00:00:00 UTC 2019

生物信息学

蛋白别名: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag

查看更多收起

DYKDDDDK Tag Monoclonal Antibody (L5), Alexa Fluor™ 488

DYKDDDDK Tag Antibody (MA1-142-A488) in ICC/IF

产品信息

MA1-142-A488

免疫印迹 (WB)

免疫组化 (IHC)

免疫组织化学（多聚甲醛固定） (IHC (PFA))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

免疫沉淀 (IP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

激发/发射光谱

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!