Invitrogen

Decorin Polyclonal Antibody

4 Published Figures

6 References

View all (29) Decorin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Published Figures (4)

Decorin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 7

Decorin Antibody (PA5-27370) in IHC (P)

Immunohistochemical analysis of paraffin-embedded TOV112D xenograft, using Decorin (Product # PA5-27370) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Decorin Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

Product Details

PA5-27370

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent

in situ PLA (PLA)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Horse, Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 192 of Human Decorin

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 40% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2544846

Product Specific Information

Recommended positive controls: A549, H1299.

Predicted reactivity: Mouse (82%), Rat (80%), Dog (91%), Pig (88%), Rabbit (90%), Sheep (86%), Chimpanzee (100%), Bovine (87%), Guinea pig (87%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Decorin is a member of the small, leucine-rich proteoglycan (SLRP) family along with biglycan. Found in the interterritorial region of cartilage, decorin interacts with collagen and other matrix proteins to regulate the cartilaginous matrix. It has been shown to interact with fibronectin, thrombospondin, C1q, epidermal growth factor receptor (EGFR), and TGF-b. It is believed that decorin also plays a role in the cell cycle of chondrocytes. The decorin neoepitope fragment has a sequence of PKTLQE, and is generated by MMP-3 or ADAMTS-4 cleavage at Glu154-Leu155 of decorin core protein.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cell-Laden Marine Gelatin Methacryloyl Hydrogels Enriched with Ascorbic Acid for Corneal Stroma Regeneration.

Bioengineering (Basel, Switzerland)

Alves AL,Carvalho AC,Machado I,Diogo GS,Fernandes EM,Castro VIB,Pires RA,Vázquez JA,Pérez-Martín RI,Alaminos M,Reis RL,Silva TH

Published figure using Decorin polyclonal antibody (Product # PA5-27370) in Immunocytochemistry

Wed Jan 04 00:00:00 UTC 2023

Characterization of the Proteins Secreted by Equine Muscle-Derived Mesenchymal Stem Cells Exposed to Cartilage Explants in Osteoarthritis Model.

Stem cell reviews and reports

Dechêne L,Colin M,Demazy C,Fransolet M,Niesten A,Arnould T,Serteyn D,Dieu M,Renard P

PA5-27370 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to suggest that muscle-derived MSCs could reduce the catabolic effect of TNFα and IL-1β on cartilage explant by decreasing the secretion and activity of matrix metalloproteinase 3 and increasing the decorin secretion.

Wed Feb 01 00:00:00 UTC 2023

Capture and Detection of Circulating Glioma Cells Using the Recombinant VAR2CSA Malaria Protein.

Cells

Bang-Christensen SR,Pedersen RS,Pereira MA,Clausen TM,Løppke C,Sand NT,Ahrens TD,Jørgensen AM,Lim YC,Goksøyr L,Choudhary S,Gustavsson T,Dagil R,Daugaard M,Sander AF,Torp MH,Søgaard M,Theander TG,Østrup O,Lassen U,Hamerlik P,Salanti A,Agerbæk MØ

PA5-27370 was used in Proximity Ligation Assay (PLA) to present a method for enriching and staining CTCs from glioma patients.

Wed Aug 28 00:00:00 UTC 2019

Leptospira interrogans causes quantitative and morphological disturbances in adherens junctions and other biological groups of proteins in human endothelial cells.

PLoS neglected tropical diseases

Sato H,Coburn J

Published figure using Decorin polyclonal antibody (Product # PA5-27370) in Immunofluorescence

Sat Jul 01 00:00:00 UTC 2017

Placental Sequestration of Plasmodium falciparum Malaria Parasites Is Mediated by the Interaction Between VAR2CSA and Chondroitin Sulfate A on Syndecan-1.

PLoS pathogens

Ayres Pereira M,Mandel Clausen T,Pehrson C,Mao Y,Resende M,Daugaard M,Riis Kristensen A,Spliid C,Mathiesen L,E Knudsen L,Damm P,G Theander T,R Hansson S,A Nielsen M,Salanti A

PA5-27370 was used in immunocytochemistry, immunohistochemistry - paraffin section, and proximity ligation assay characterize the interaction between VAR2CSA and chondroitin sulfate proteoglycans

Mon Aug 01 00:00:00 UTC 2016

Placental Sequestration of Plasmodium falciparum Malaria Parasites Is Mediated by the Interaction Between VAR2CSA and Chondroitin Sulfate A on Syndecan-1.

PLoS pathogens

Ayres Pereira M,Mandel Clausen T,Pehrson C,Mao Y,Resende M,Daugaard M,Riis Kristensen A,Spliid C,Mathiesen L,E Knudsen L,Damm P,G Theander T,R Hansson S,A Nielsen M,Salanti A

PA5-27370 was used in immunocytochemistry, immunohistochemistry - paraffin section, and proximity ligation assay characterize the interaction between VAR2CSA and chondroitin sulfate proteoglycans

Mon Aug 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: Bone proteoglycan II; Decorin; dermatan sulphate proteoglycans II; Dermatan sulphate proteoglycans II (DSPG2); PG-S2; PG40; proteoglycan core protein; Small leucine rich protein 1B; small leucine-rich protein 1B

View more View less

Gene Aliases: CSCD; DCN; DSPG2; PG40; PGII; PGS2; SLRR1B

View more View less

UniProt ID: (Human) P07585

View more View less

Entrez Gene ID: (Human) 1634

View more View less

Function(s)

protein kinase inhibitor activity protein binding collagen binding glycosaminoglycan binding poly(A) RNA binding protein N-terminus binding extracellular matrix binding

Process(es)

kidney development placenta development negative regulation of protein kinase activity skeletal muscle tissue development aging response to mechanical stimulus organ morphogenesis positive regulation of autophagy negative regulation of endothelial cell migration positive regulation of phosphatidylinositol 3-kinase signaling positive regulation of macroautophagy negative regulation of angiogenesis cytokine-mediated signaling pathway peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan extracellular matrix disassembly extracellular matrix organization glycosaminoglycan metabolic process chondroitin sulfate biosynthetic process chondroitin sulfate catabolic process dermatan sulfate biosynthetic process response to lipopolysaccharide wound healing positive regulation of transcription from RNA polymerase II promoter negative regulation of JAK-STAT cascade positive regulation of mitochondrial depolarization positive regulation of mitochondrial fission negative regulation of vascular endothelial growth factor signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

Decorin Polyclonal Antibody

Decorin Antibody (PA5-27370) in IHC (P)

Product Details

PA5-27370

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

in situ PLA (PLA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!