Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
产品信息
200-4361-0100
产品规格
种属反应
Bovine
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Deoxyribonuclease I [Bovine Pancreas]
偶联物
形式
Lyophilized
浓度
1 mg/mL
纯化类型
Ion-exchange chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA
内含物
0.01% gentamicin sulfate
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Deoxyribonuclease I is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Deoxyribonuclease I [Bovine Pancreas]. Cross reactivity against Deoxyribonuclease I from other tissues and species may occur but have not been specifically determined.
靶标信息
Internucleosomal DNA fragmentation following the activation of endonucleases is the common end point of apoptosis. DNase I, a Ca2+/Mg2+-dependent endonuclease ubiquitously expressed in mammalian tissues, has been implicated to mediate internucleosomal DNA degradation in human cells undergoing apoptosis. DNase I is highly polymorphic, and at least six alleles of DNase I are known. DNase II, the ubiquitously expressed acidic deoxyribonuclease, acts downstream of caspase activation and may also induce DNA digestion during apoptosis. DNase I cleaves DNA to 5'-phosphodinucleotide and 5'-phospho- oligonucleotide end-products, whereas DNase II cleaves DNA to 3'-phosphomononucleotide and 3'-phosphooligonucleotide end-products. The mechanism by which DNase II cuts DNA is similar to DNase I, which produces nicks rather than double-strand cuts. DNase II is usually present in cytoplasm of epithelial cells, but it appears concentrated in the nuclei of lens fibers. In contrast, DNase I is always concentrated in nuclei of epithelial and fiber cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: deoxyribonuclease; Deoxyribonuclease 1; Deoxyribonuclease I; Deoxyribonuclease-1; Deoxyribonuclease1; DeoxyribonucleaseI; DNASE 1; DNase I; DNase I lysosomal; DNL 1; FLJ38093
基因别名: DNASE1; DNL1
UniProt ID: (Bovine) P00639
Entrez Gene ID: (Bovine) 282217
