Invitrogen

Desmin Recombinant Rabbit Monoclonal Antibody (ARC0235)

Desmin Antibody in Immunocytochemistry (ICC/IF)

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Desmin Antibody (MA5-38096) in ICC/IF

Immunofluorescence analysis of Desmin was performed using 70% confluent log phase RD cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Desmin Recombinant Rabbit Monoclonal Antibody (ARC0235) (Product # MA5-38096) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor... View More

Desmin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

MA5-38096

应用

建议稀释比

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免疫印迹 (WB)

1:1,000-1:5,000

免疫组化（石蜡） (IHC (P))

1:1,000-1:5,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

ARC0235

抗原

A synthetic peptide corresponding to a sequence within amino acids 371-470 of human Desmin (P17661)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2898014

产品详细信息

Positive Samples: C2C12, RD, Mouse lung, Mouse heart, Rat heart

Immunogen sequence: EEEIRHLKDE MARHLREYQD LLNVKMALDV EIATYRKLLE GEESRINLPI QTYSALNFRE TSPEQRGSEV HTKKTVMIKT IETRDGEVVS EATQQQHEVL

靶标信息

Desmin (approximately 53 kDa) exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development of, for example, skeletal muscle cell differentiation. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.

仅用于科研。不用于诊断过程。未经明确授权不得转售。