Invitrogen

Doublecortin Monoclonal Antibody (2G5)

Doublecortin Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 7

Doublecortin Antibody (MA5-17066) in ICC/IF

Immunofluorescence analysis of HepG2 cells using DCX monoclonal antibody (Product # MA5-17066) (Green). Blue: DRAQ5 fluorescent DNA dye.

Doublecortin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Doublecortin Antibody in Western Blot (WB)

Doublecortin Antibody in Flow Cytometry (Flow)

Doublecortin Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

Product Image Gallery

产品信息

MA5-17066

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:200-1:1,000

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:200-1:1,000

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

1:200-1:400

酶联免疫吸附实验 (ELISA)

1:10,000

产品规格

种属反应

Human, Mouse

已发表种属

Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

2G5

抗原

Purified recombinant fragment of human DCX (Uniprot: aa 281-335) expressed in E. Coli (NP_000546: aa 462-411).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2538537

产品详细信息

MA5-17066 targets DCX in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human samples.

The MA5-17066 immunogen is purified recombinant fragment of human DCX (amino acids: 362-411) expressed in E. Coli.

MA5-17066 detects DCX which has a predicted molecular weight of approximately 49.3kDa.

靶标信息

In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. DCX is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. It contains two doublecortin domains, which bind microtubules. In addition, this protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in the gene encoding DCX are a cause of X-linked lissencephaly.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Neuroprotection of N-benzyl Eicosapentaenamide in Neonatal Mice Following Hypoxic-Ischemic Brain Injury.

Molecules (Basel, Switzerland)

Jiao M,Dong Q,Zhang Y,Lin M,Zhou W,Liu T,Yuan B,Yin H

MA5-17066 was used in Immunohistochemistry (Paraffin) to investigate the neuroprotective role of N-benzyl eicosapentaenamide (NB-EPA) in hypoxic-ischemic brain injury.

Sat May 22 00:00:00 UTC 2021

Neuroprotection of N-benzyl Eicosapentaenamide in Neonatal Mice Following Hypoxic-Ischemic Brain Injury.

Molecules (Basel, Switzerland)

Jiao M,Dong Q,Zhang Y,Lin M,Zhou W,Liu T,Yuan B,Yin H

MA5-17066 was used in Immunohistochemistry (Paraffin) to investigate the neuroprotective role of N-benzyl eicosapentaenamide (NB-EPA) in hypoxic-ischemic brain injury.

Sat May 22 00:00:00 UTC 2021

Neuroprotection of N-benzyl Eicosapentaenamide in Neonatal Mice Following Hypoxic-Ischemic Brain Injury.

Molecules (Basel, Switzerland)

Jiao M,Dong Q,Zhang Y,Lin M,Zhou W,Liu T,Yuan B,Yin H

MA5-17066 was used in Immunohistochemistry (Paraffin) to investigate the neuroprotective role of N-benzyl eicosapentaenamide (NB-EPA) in hypoxic-ischemic brain injury.

Sat May 22 00:00:00 UTC 2021

A Combinatorial MAP Code Dictates Polarized Microtubule Transport.

Developmental cell

Monroy BY,Tan TC,Oclaman JM,Han JS,Simó S,Niwa S,Nowakowski DW,McKenney RJ,Ori-McKenney KM

MA5-17066 was used in Immunocytochemistry-immunoflourescence to propose a general "MAP code" that has the capacity to strongly bias directed movement along microtubules and helps elucidate the intricate intracellular sorting observed in highly polarized cells such as neurons.

Mon Apr 06 00:00:00 UTC 2020

生物信息学

蛋白别名: doublecortex; Doublin; Lis-X; LIS-XD; Lissencephalin-X; lissencephaly, X-linked (doublecortin); Neuronal migration protein doublecortin; OTTHUMP00000023860; OTTHUMP00000216316

查看更多收起

基因别名: DBCN; Dbct; DC; Dcn; DCX; LISX; SCLH; XLIS

查看更多收起

UniProt ID: (Human) O43602, (Mouse) O88809

查看更多收起

Entrez Gene ID: (Human) 1641, (Mouse) 13193

查看更多收起

功能

calmodulin-dependent protein kinase activity protein binding calmodulin binding microtubule binding calcium-dependent protein serine/threonine kinase activity protein kinase binding

参与通路