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Dynactin 1 Antibody (PA5-37360) in WB
Western blot was performed using Anti-Dynactin 1 Polyclonal Antibody (Product # PA5-37360) and a 141 kDa band corresponding to DCTN1 was observed across all the cell lines tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), Jurkat (Lane 2), SH-SY5Y (Lane 3) and U-87 MG (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the... View More
产品信息
PA5-37360
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant full length human DCTN1
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.2, with 50% glycerol
内含物
0.02% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2554027
产品详细信息
This antibody detects endogenous protein at a molecular weight of 150 kDa.
Purity is >95% by SDS-PAGE.
靶标信息
This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 150 kDa dynein-associated polypeptide; DAP 150; DAP-150; DAP150; DCTN 1; DP 150; DP-150; DP150; dynactin 1 (p150, glued homolog, Drosophila); dynactin 1, retrograde axonal transport; Dynactin subunit 1; Dynactin1; HMN7B; OTTHUMP00000202491; p135; p150 glued; p150 glued homolog; p150-glued; p150glued
基因别名:
AL022633; DAP-150; DCTN1; DP-150; Glued; P135; p150; p150
UniProt ID: (Human) Q14203, (Rat) P28023, (Mouse) O08788
Entrez Gene ID: (Human) 1639, (Rat) 29167, (Mouse) 13191
G2/M transition of mitotic cell cycle
ER to Golgi vesicle-mediated transport
mitotic nuclear division
nervous system development
transport along microtubule
antigen processing and presentation of exogenous peptide antigen via MHC class II
melanosome transport
IRE1-mediated unfolded protein response
retrograde transport, endosome to Golgi
microtubule-based process
metabolic process
cytoplasmic microtubule organization
positive regulation of intracellular protein transport
transport
