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Dynamin 3 Antibody (PA1-662) in ICC/IF
Immunofluorescence analysis of Dynamin III was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Dynamin III Rabbit Polyclonal Antibody (Product # PA1-662) at 2 µg/mL in 0.1% BSA and incubated for 3 hours ... View More
产品信息
PA1-662
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic Peptide: C P(623) D K S F T E N D E N G Q A E N F(639)
偶联物
形式
Liquid
浓度
Conc. Not Determined
纯化类型
Ammonium sulfate precipitation
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2093694
产品详细信息
PA1-662 detects dynamin III (Dyn3) from rat, mouse, and human tissues and cells.
PA1-662 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~100 kDa protein representing Dyn3 from HeLa cell lysate.
PA1-662 immunizing peptide corresponds to amino acid residues 623-639 from rat Dyn3. PA1-662 immunizing peptide (Cat. # PEP-057) is available for use in neutralization and control experiments.
靶标信息
The dynamins are a family of 100 kDa GTPases transcribed from at least three separate genes. At least four mRNA splice variants for each dynamin have been described. Dynamins contain several conserved regions including the conserved, amino-terminal GTPase domain, a centrally located membrane-binding plekstrin homology domain (PHD), and a coiled-coil region located in front of a proline-rich domain (PRD). The PRD is thought to mediate interactions between dynamin and numerous other cellular proteins. Dynamin I is expressed exclusively in neurons, dynamin II is ubiquitously expressed, and dynamin III is thought to be restricted to expression in the brain, testis, heart, and lung. The dynamins participate in the cellular process of clathrin-mediated and fluid-phase endocytosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Dyna III; dynamin family member; Dynamin III; Dynamin, testicular; Dynamin-3; T-dynamin; testicular dynamin
基因别名: 9630020E24Rik; AW061159; B230343F03Rik; C530045C17; DNM3; Dyn3; Dyna III; dynamin-2; KIAA0820; mKIAA0820
UniProt ID: (Human) Q9UQ16, (Mouse) Q8BZ98, (Rat) Q08877
Entrez Gene ID: (Human) 26052, (Mouse) 103967, (Rat) 171574
