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Dynein Antibody (14-9772-80) in ICC/IF
Immunocytochemistry of methanol-fixed and permeabilized HeLa cells stained with 1 µg/mL of Mouse IgG2b K Isotype Control Purified (left) or 1 µg/mL of Anti-Dynein Purified (right) followed by F(ab')2 Anti-Mouse IgG eFluor® 570.Nuclei are stained with DAPI.
产品信息
14-9772-80
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Fruit fly,
Human,
Mouse,
Sheep,
Pig,
Rat,
Xenopus
已发表种属
Fruit fly,
Mouse
宿主/亚型
Mouse
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
74.1
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% Sodium Azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2573019
产品详细信息
The monoclonal antibody 74.1 recognizes all known forms of the 74 kDa intermediate chain subunit of mammalian cytoplasmic dynein. Cytoplasmic dynein is a microtubule-stimulated ATPase responsible for movement of membrane-bound organelles in a directed manner toward the minus end of microtubules, commonly found at the centrosome. Dynein is responsible for the transport of lysosomes, endosomes, phagosomes, peroxisomes, mitochondria, and vesicles from the endoplasmic reticulum destined for the Golgi. During mitosis, dynein is responsible for positioning the spindle apparatus as well as being localized to the kinetochores to facilitate chromosome movement.The 74.1 antibody recognizes human, mouse, rat, bovine, sheep, Xenopus, porcine, and Drosophila dynein intermediate chain.
This 74.1 antibody has been tested by immunocytochemistry of methanol-fixed and permeabilized cells and can be used at less than or equal to 1 µg/mL. This 74.1 antibody has been tested by western blot of reduced and non-reduced HeLa cell lysate and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
The directed movement of membrane-bounded organelles is an important process for various cellular functions including membrane transport, secretion, and axonal transport. Cytoplasmic dynein, a ubiquitous minus end-directed microtubule-based motor protein, is believed to be responsible for the retrograde transport of membranous organelles from the synapse to the cell body in fast axonal transport. The cytoplasmic dynein complex is composed of two ~530 kDa heavy chains, and intermediate chains of ~74 kDa and ~53-59 kDa.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 8 kDa dynein light chain; 8 kDa dynein light chain b; 8kD LC; 8kDa LC; cytoplasmic dynein light polypeptide; cytoplasmic light-chain dynein; cytosolic DH IC-1; DLC8; DLC8b; DNCL1; dynein LC8; Dynein light chain 1, cytoplasmic; Dynein light chain 2, cytoplasmic; Dynein light chain LC8-type 1; Dynein light chain LC8-type 2; dynein light chain-2; dynein, cytoplasmic, light chain 1; dynein, cytoplasmic, light peptide; dynein, cytoplasmic, light polypeptide 1; dynein, light chain, LC8-type 1; dynein, light chain, LC8-type 2; LC8; PIN; mPIN; PIN; Protein inhibitor of neuronal nitric oxide synthase; protein inhibitor of nitric oxide synthase; radial spoke 22 homolog
基因别名: 1700064A15Rik; 6720463E02Rik; 8kDLC; C87222; CDLC2; DLC1; DLC2; DLC8; DLC8b; DNCL1; DNCL1B; DNCLC1; DYNLL1; DYNLL2; HDLC1; LC8; LC8a; PIN; RSPH22
G2/M transition of mitotic cell cycle
transcription, DNA-templated
regulation of transcription, DNA-templated
ER to Golgi vesicle-mediated transport
apoptotic process
female gamete generation
anatomical structure morphogenesis
viral process
macroautophagy
antigen processing and presentation of exogenous peptide antigen via MHC class II
substantia nigra development
actin cytoskeleton organization
intraciliary retrograde transport
negative regulation of phosphorylation
cilium morphogenesis
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
microtubule-based process
synaptic target recognition
nitric oxide biosynthetic process
transport
neurotransmitter metabolic process
negative regulation of catalytic activity
regulation of catalytic activity
microtubule-based movement
negative regulation of nitric oxide biosynthetic process
metabolic process
intracellular transport
