Invitrogen

E-cadherin Recombinant Rabbit Monoclonal Antibody (5H6L18)

E-cadherin Antibody in Immunocytochemistry (ICC/IF)

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E-cadherin Antibody (701134) in ICC/IF

Immunofluorescent analysis of E-cadherin/CD324 was performed on 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with E-cadherin/CD324 Recombinant Rabbit Monoclonal Antibody (Product # 701134) at a dilution of 1:500 in 1% BSA and incubated for 3 hours at room temperature and then... View More

E-cadherin Antibody in Western Blot (WB)

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产品信息

701134

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:5,000

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免疫细胞化学 (ICC/IF)

1:100-1:1,000

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酶联免疫吸附实验 (ELISA)

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产品规格

种属反应

Human

已发表种属

Fish, Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

5H6L18

抗原

Peptide corresponding to amino acids 668-77 of human E-cadherin (epithelial)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2532405

产品详细信息

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Integrative Single-Cell Analysis Reveals Transcriptional and Epigenetic Regulatory Features of Clear Cell Renal Cell Carcinoma.

Cancer research

Yu Z,Lv Y,Su C,Lu W,Zhang R,Li J,Guo B,Yan H,Liu D,Yang Z,Mi H,Mo L,Guo Y,Feng W,Xu H,Peng W,Cheng J,Nan A,Mo Z

Published figure using E-cadherin recombinant monoclonal antibody (Product # 701134) in Western Blot

Thu Mar 02 00:00:00 UTC 2023

14-3-3 and Smad2/3 are crucial mediators of atypical-PKCs: Implications for neuroblastoma progression.

Frontiers in oncology

Breedy S,Ratnayake WS,Lajmi L,Hill R,Acevedo-Duncan M

Published figure using E-cadherin recombinant monoclonal antibody (Product # 701134) in Western Blot

Mon Sep 18 00:00:00 UTC 2023

From a cell model to a fish trial: Immunomodulatory effects of heat-killed Lactiplantibacillus plantarum as a functional ingredient in aquafeeds for salmonids.

Frontiers in immunology

Rocha SDC,Lei P,Morales-Lange B,Mydland LT,Øverland M

701134 was used in ELISA to show that HK L-137 can modulate physiological response of Atlantic salmon, making fish more robust against stressful conditions during production.

Fri Mar 31 00:00:00 UTC 2023

Epigenetic network of EZH2/SFRP1/Wnt in the epithelial-mesenchymal transition of laryngeal carcinoma cells.

Neoplasma

Chen W,Wang HT,Ji JF,Wang ZY,Shi T,Wu MH,Yu PJ

701134 was used in Western Blotting to explore the role and mechanism of EZH2 in the epithelial-mesenchymal transition (EMT) of laryngeal carcinoma (LGC) cells.

Sun May 01 00:00:00 UTC 2022

Atypical PKCs activate Vimentin to facilitate prostate cancer cell motility and invasion.

Cell adhesion & migration

Ratnayake WS,Apostolatos CA,Breedy S,Dennison CL,Hill R,Acevedo-Duncan M

701134 was used in Western Blotting to understand the interaction of atypical protein kinase C with Vimentin and their role in metastasis of prostate cancer cells.

Wed Dec 01 00:00:00 UTC 2021

Atypical PKCs activate Vimentin to facilitate prostate cancer cell motility and invasion.

Cell adhesion & migration

Ratnayake WS,Apostolatos CA,Breedy S,Dennison CL,Hill R,Acevedo-Duncan M

701134 was used in Western Blotting to understand the interaction of atypical protein kinase C with Vimentin and their role in metastasis of prostate cancer cells.

Wed Dec 01 00:00:00 UTC 2021

Endothelial Differentiation G Protein-Coupled Receptor 5 Plays an Important Role in Induction and Maintenance of Pluripotency.

Stem cells (Dayton, Ohio)

Neganova I,Cotts L,Banks P,Gassner K,Shukurov A,Armstrong L,Ladds G,Lako M

701134 was used in Western Blotting to study the role of endothelial differentiation G protein-coupled receptor 5 in the maintenance and acquisition of pluripotency.

Fri Mar 01 00:00:00 UTC 2019

Analysis of PKC-ζ protein levels in normal and malignant breast tissue subtypes.

Oncology letters

Smalley T,Islam SMA,Apostolatos C,Apostolatos A,Acevedo-Duncan M

701134 was used in Western Blotting to analyse atypical protein kinase C-ζ protein levels in normal and malignant breast tissue subtypes.

Fri Feb 01 00:00:00 UTC 2019

Rainbow Trout (Oncorhynchus Mykiss) Intestinal Epithelial Cells as a Model for Studying Gut Immune Function and Effects of Functional Feed Ingredients.

Frontiers in immunology

Wang J,Lei P,Gamil AAA,Lagos L,Yue Y,Schirmer K,Mydland LT,Øverland M,Krogdahl Å,Kortner TM

701134 was used in Western Blotting to evaluate the effects of lipopolysaccharide and three functional feed ingredients in rainbow trout intestinal epithelial cell line cells grown on conventional culture plates and transwell membranes.

Mon Jan 06 00:00:00 UTC 2020

HDAC inhibitors, trichostatin A and valproic acid, increase E‑cadherin and vimentin expression but inhibit migration and invasion of cholangiocarcinoma cells.

Oncology reports

Wang JH,Lee EJ,Ji M,Park SM

701134 was used in Western Blotting to conclude that treatment of biliary cancer with HDAC inhibitors, trionchostatin A and valproic acid, should be should be considered with caution due to their abilities to augment both E-cadherin and vimentin expression in cholangiocarcinoma cells.

Sun Jul 01 00:00:00 UTC 2018

Two novel atypical PKC inhibitors; ACPD and DNDA effectively mitigate cell proliferation and epithelial to mesenchymal transition of metastatic melanoma while inducing apoptosis.

International journal of oncology

Ratnayake WS,Apostolatos AH,Ostrov DA,Acevedo-Duncan M

Wed Nov 01 00:00:00 UTC 2017

Cisplatin promotes mesenchymal-like characteristics in osteosarcoma through Snail.

Oncology letters

Fang S,Yu L,Mei H,Yang J,Gao T,Cheng A,Guo W,Xia K,Liu G

701134 was used in immunocytochemistry to test if chemotherapy affects the epithelial-mesenchymal transition of osteosarcoma cells

Thu Dec 01 00:00:00 UTC 2016

生物信息学

蛋白别名: cadherin 1, E-cadherin (epithelial); cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin e; Cadherin-1; cadherin-E; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; cell-CAM 120/80; E-Cadherin; E-cadherin 1; Ecadherin; Epithelial cadherin; Uvomorulin

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基因别名: Arc-1; CD324; CDH1; CDHE; ECAD; LCAM; UVO

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UniProt ID: (Human) P12830

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Entrez Gene ID: (Human) 999

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功能