Invitrogen

E-cadherin Monoclonal Antibody (ECCD-1)

E-cadherin Antibody in Western Blot (WB)

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E-cadherin Antibody (13-1800) in WB

Frontiers in molecular neuroscience 2022 - No change was found in the protein expression level of G-actin. Protein expression level of Cx26, beta-actin (A) , and gamma-actin (E) in P0 control and P0 KD groups. beta-actin and gamma-actin protein expression level in cochlear of Cx26 KD mouse models. The ratio of G-actin/F-actin in P0 control and P0 KD group (B) respectively. Western blotting F-actin and G-actin in Western blot results of protein expre... View More

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产品信息

13-1800

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看4篇已发表文章 4篇已发表文章

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

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免疫细胞化学 (ICC/IF)

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中和实验 (Neu)

查看3篇已发表文章 3篇已发表文章

抑制试剂 (IA)

200 µg/mL

其他PubMed (Misc)

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产品规格

种属反应

Mouse

已发表种属

Fish, Human, Mouse, Rat, Tag

宿主/亚型

Rat / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

ECCD-1

抗原

Mouse teratocarcinoma cell line F9.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

2.0 mg/mL

纯化类型

purified

保存液

PBS, pH 7.4, with 1% BSA

内含物

no preservative

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533004

产品详细信息

Source: Monoclonal antibody was obtained by fusing the P3-X63-Ag8 mouse myeloma cell line with spleen cells of Wistar rat after immunization with mouse teratocarcinoma cell line F9.

Specificity: This antibody specifically reacts with mouse E-cadherin. This antibody inhibits E-cadherin dependent cell-cell contact.

Cross reactivity: This antibody does not react with human, bovine, chicken or dog E-cadherin.

Storage: 4°C This product does not contain preservative. The stock solution (2.0 mg/mL) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles.

Reconstitution: Dissolve the lyophilized antibody in 50 µL of distilled water (final concentration: 2.0 mg/mL). This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 µL or more of the Dilution solution. Be sure to store the antibody at a minimum concentration of 2.0 mg/mL. A lower antibody concentration may result in decreased stability. Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C.

靶标信息

E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

F-Actin Dysplasia Involved in Organ of Corti Deformity in Gjb2 Knockdown Mouse Model.

Frontiers in molecular neuroscience

Liu XZ,Jin Y,Chen S,Xu K,Xie L,Qiu Y,Wang XH,Sun Y,Kong WJ

13-1800 was used in Immunohistochemistry and Western Blotting to indicate that Cx26 is involved in the maturation of the cytoskeleton during the development of the OC at the early postnatal stage.

Wed Mar 30 00:00:00 UTC 2022

F-Actin Dysplasia Involved in Organ of Corti Deformity in Gjb2 Knockdown Mouse Model.

Frontiers in molecular neuroscience

Liu XZ,Jin Y,Chen S,Xu K,Xie L,Qiu Y,Wang XH,Sun Y,Kong WJ

13-1800 was used in Immunohistochemistry and Western Blotting to indicate that Cx26 is involved in the maturation of the cytoskeleton during the development of the OC at the early postnatal stage.

Wed Mar 30 00:00:00 UTC 2022

Identification of MYC-Dependent Transcriptional Programs in Oncogene-Addicted Liver Tumors.

Cancer research

Kress TR,Pellanda P,Pellegrinet L,Bianchi V,Nicoli P,Doni M,Recordati C,Bianchi S,Rotta L,Capra T,Ravà M,Verrecchia A,Radaelli E,Littlewood TD,Evan GI,Amati B

131800 was used in immunohistochemistry - paraffin section to find MYC-dependent regulatory events present in a fully developed carcinoma

Wed Jun 15 00:00:00 UTC 2016

Lgr5 Marks Neural Crest Derived Multipotent Oral Stromal Stem Cells.

Stem cells (Dayton, Ohio)

Boddupally K,Wang G,Chen Y,Kobielak A

13-1800 was used in immunohistochemistry - frozen section to elucidate neural crest derived multipotent oral stromal stem cells marked by Lgr5

Tue Mar 01 00:00:00 UTC 2016

E-cadherin-expressing feeder cells promote neural lineage restriction of human embryonic stem cells.

Stem cells and development

Moore RN,Cherry JF,Mathur V,Cohen R,Grumet M,Moghe PV

13-1800 was used in blocking or activating experiment and immunocytochemistry to describe a protocol to differentiate hESCs into neural stem cells, which can then be differentiated into neuronal restricted cells.