Invitrogen

E-cadherin Monoclonal Antibody (NCH-38)

E-cadherin Antibody in Immunocytochemistry (ICC/IF)

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E-cadherin Antibody (MA5-12547) in ICC/IF

Immunofluorescence analysis of E-cadherin was performed using 70 percent confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with E-cadherin Monoclonal Antibody (NCH-38) (Product # MA5-12547) at 1:200 in 0.1% BSA, incubated at 4 ... View More

E-cadherin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

E-cadherin Antibody in Western Blot (WB)

E-cadherin Antibody in Immunohistochemistry (IHC)

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产品信息

MA5-12547

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:250

免疫组化 (IHC)

查看9篇已发表文章 9篇已发表文章

免疫组化（石蜡） (IHC (P))

1:5-1:10

免疫细胞化学 (ICC/IF)

1:200

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse, Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

NCH-38

抗原

E-cadherin and GST recombinant protein

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

tissue culture supernatant

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Wet ice

AB_10982676

产品详细信息

MA5-12547 targets E-Cadherin in IHC (P) applications and shows reactivity with Human samples.

The MA5-12547 immunogen is e-cadherin and GST recombinant protein.

靶标信息

E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Ankyrin-G regulated epithelial phenotype is required for mouse lens morphogenesis and growth.

Developmental biology

Rasiah PK,Maddala R,Bennett V,Rao PV

MA5-12547 was used in Immunohistochemistry-immunofluorescence to study the function of scaffolding protein ankyrin-G (AnkG) in lens development of mice.

Fri Feb 01 00:00:00 UTC 2019

Overexpression and Tyr421-phosphorylation of cortactin is induced by three-dimensional spheroid culturing and contributes to migration and invasion of pancreatic ductal adenocarcinoma (PDAC) cells.

Cancer cell international

Stock K,Borrink R,Mikesch JH,Hansmeier A,Rehkämper J,Trautmann M,Wardelmann E,Hartmann W,Sperveslage J,Steinestel K

MA5-12547 was used in Western Blotting to examine the biological role of cortactin in the progression of pancreatic ductal adenocarcinoma.

Fri Oct 06 00:00:00 UTC 2023

Clear Cell Renal Cell Carcinoma is linked to Epithelial-to-Mesenchymal Transition and to Fibrosis.

Physiological reports

Landolt L,Eikrem Ø,Strauss P,Scherer A,Lovett DH,Beisland C,Finne K,Osman T,Ibrahim MM,Gausdal G,Ahmed L,Lorens JB,Thiery JP,Tan TZ,Sekulic M,Marti HP

Published figure using E-cadherin monoclonal antibody (Product # MA5-12547) in Immunohistochemistry

Thu Jun 01 00:00:00 UTC 2017

Acetyl-CoA carboxylase inhibitors attenuate WNT and Hedgehog signaling and suppress pancreatic tumor growth.

Oncotarget

Petrova E,Scholz A,Paul J,Sturz A,Haike K,Siegel F,Mumberg D,Liu N

MA5-12547 was used in Western Blotting to assess the potential utility of acetyl-CoA carboxylase inhibitors for pancreatic cancer treatment, and the link between upregulated de novo fatty acid synthesis in cancer cells, protein lipidation, and oncogenic signalling.

Tue Jul 25 00:00:00 UTC 2017

Malignant transformation of adenomyoepithelioma of the breast by a monophasic population: a report of two cases and review of literature.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica

Marian C,Boila A,Soanca D,Malau M,Podeanu DM,Resetkova E,Stolnicu S

MA5-12547 was used in immunohistochemistry to report on two cases of malignant transformation of adenomyoepithelioma of the breast by a monophasic population

Mon Apr 01 00:00:00 UTC 2013

Five primary human pancreatic adenocarcinoma cell lines established by the outgrowth method.

The Journal of surgical research

Rückert F,Aust D,Böhme I,Werner K,Brandt A,Diamandis EP,Krautz C,Hering S,Saeger HD,Grützmann R,Pilarsky C

MA5-12547 was used in immunohistochemistry to establish and characterize five new pancreatic adenocarcinoma cell lines

Sun Jan 01 00:00:00 UTC 2012

The expression of cytokeratin 5/6 in invasive lobular carcinoma of the breast: evidence of a basal-like subset?

Human pathology

Fadare O,Wang SA,Hileeto D

MA5-12547 was used in immunohistochemistry to investigate the expression of cytokeratin 5/6 in a subset of invasive lobular carcinoma of the breast and its prognostic significance

Sat Mar 01 00:00:00 UTC 2008

Nogo-A inhibitory peptide (NEP1-40) increases pan-cadherin expression following mild cortical contusion injury in rats.

Turkish neurosurgery

Atalay B,Bavbek M,Ozen O,Nacar A,Gülşen S,Yiğitkanli K,Caner H,Altinörs N

MA5-12547 was used in immunohistochemistry to study the effects of a Nogo-A neutralizing peptide on cadherin expression in a rat model of mild cerebral contusion

Wed Oct 01 00:00:00 UTC 2008

The expression of cytoskeleton regulatory protein Mena in colorectal lesions.

Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie

Gurzu S,Jung I,Prantner I,Ember I,Pávai Z,Mezei T

MA5-12547 was used in immunohistochemistry to investigate the expression of mammalian actin regulatory protein Ena in colorectal lesions

Thu Dec 11 00:00:00 UTC 2008

Human ovarian surface epithelial cells immortalized with hTERT maintain functional pRb and p53 expression.

Cell proliferation

Li NF,Broad S,Lu YJ,Yang JS,Watson R,Hagemann T,Wilbanks G,Jacobs I,Balkwill F,Dafou D,Gayther SA

MA5-12547 was used in immunocytochemistry to develop an imortalized human ovarian surface epithelial cell line that maintains functional pRb and p53 expression

Mon Oct 01 00:00:00 UTC 2007

Cystic hypersecretory carcinoma: rare and poorly recognized variant of intraductal carcinoma of the breast. Report of five cases.

Histopathology

Skalova A,Ryska A,Kajo K,Di Palma S,Kinkor Z,Michal M

MA5-12547 was used in immunohistochemistry to report the clinical cases of cystic hypersecretory carcinoma

Sat Jan 01 00:00:00 UTC 2005

生物信息学

蛋白别名: ARC-1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin e; Cadherin-1; cadherin-E; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; cell-CAM 120/80; E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3; E-Cadherin; E-cadherin 1; Ecadherin; Epithelial cadherin; Uvomorulin

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基因别名: AA960649; Arc-1; CD324; CDH1; CDHE; E-cad; ECAD; L-CAM; LCAM; Um; UVO

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UniProt ID: (Human) P12830, (Mouse) P09803

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Entrez Gene ID: (Human) 999, (Mouse) 12550

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功能