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E.coli Combined HCP Antibody (200-401-M61) in WB
2D Western Blot of anti-E.coli Host Cell Protein antibody. Load: 35 µg Total HCP. Primary antibody: Rabbit anti-HCP antibody at 1:200 for overnight at 4°C. Secondary antibody: Goat anti-rabbit secondary antibody at 1:10,000 for 30 min at RT. Block: MB-070 for 1 hour at RT.
产品信息
200-401-M61
产品规格
种属反应
Bacteria
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Anti-Host Cell Proteins Antibody was prepared by repeated immunizations with a full range of high molecular weight (HMW) and low molecular weight (LMW) proteins from E.Coli (BL21).
偶联物
形式
Liquid
浓度
0.82 mg/mL
纯化类型
Protein A
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Host Cell Protein A purified antibody is directed against high molecular weight and low molecular weight E.coli Host Cell Proteins. Reactivity is directed against E.coli Host Cell Proteins. Cross-reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.
靶标信息
Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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