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E2F1 Antibody (PA5-27365) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of E2F1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: E2F1 Polyclonal Antibody (Product # PA5-27365) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
产品信息
PA5-27365
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 43 and 217 of Human E2F1
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
0.1M tris glycine, pH 7, with 10% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2544841
产品详细信息
Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, NIH-3T3, E2F1-transfected 293T.
Predicted reactivity: Mouse (96%), Rat (97%), Chicken (86%), Bovine (96%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: E2F-1; PBR3; PRB-binding protein E2F-1; RBAP-1; RBBP-3; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; Transcription factor E2F1
基因别名: E2F-1; E2F1; RBAP1; RBBP3; RBP3
UniProt ID: (Human) Q01094
Entrez Gene ID: (Human) 1869
DNA damage checkpoint
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
spermatogenesis
intrinsic apoptotic signaling pathway in response to DNA damage
positive regulation of gene expression
forebrain development
anoikis
negative regulation of DNA binding
negative regulation of fat cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of fibroblast proliferation
mRNA stabilization
negative regulation of fat cell proliferation
cellular response to fatty acid
cellular response to hypoxia
cellular response to xenobiotic stimulus
negative regulation of transcription involved in G1/S transition of mitotic cell cycle
intrinsic apoptotic signaling pathway by p53 class mediator
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
lens fiber cell apoptotic process
cellular response to nerve growth factor stimulus
regulation of G1/S transition of mitotic cell cycle
