Invitrogen

E2F1 Monoclonal Antibody (17E2)

E2F1 Antibody in Immunocytochemistry (ICC/IF)

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E2F1 Antibody (MA1-23202) in ICC/IF

Immunofluorescence analysis of E2F1 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with E2F1 Monoclonal Antibody (17E2) (Product # MA1-23202) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Pro... View More

E2F1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

E2F1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

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E2F1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Hep G2 cells were transfected with E2F1 siRNA and reduction of signal was observed in Western Blot using E2F1 Monoclonal Antibody (17E2) (Product # MA1-23202). {KD}

产品信息

MA1-23202

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-5 µg/mL

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免疫组化（石蜡） (IHC (P))

Assay-dependent

免疫细胞化学 (ICC/IF)

5 µg/ml

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免疫沉淀 (IP)

Assay-dependent

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

17E2

抗原

Transactivation/retinoblastoma protein binding domain (a.a. 332-390) of E2F-1 expressed in E. coli.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7, with 20% glycerol

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_558714

产品详细信息

A suggested positive control is E2F-1 transfected Soas2 cells.

靶标信息

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

PRR11 promotes cell proliferation by regulating PTTG1 through interacting with E2F1 transcription factor in pan-cancer.

Frontiers in molecular biosciences

Zhang H,He Z,Qiu L,Wei J,Gong X,Xian M,Chen Z,Cui Y,Fu S,Zhang Z,Hu B,Zhang X,Lin S,Du H

MA1-23202 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to find that PRR11 served as an oncogene in pan-cancer and influences via cell cycle progression by PTTG1 expression regulation, through TF E2F1 interaction.

Wed Sep 07 00:00:00 UTC 2022

PRR11 promotes cell proliferation by regulating PTTG1 through interacting with E2F1 transcription factor in pan-cancer.

Frontiers in molecular biosciences

Zhang H,He Z,Qiu L,Wei J,Gong X,Xian M,Chen Z,Cui Y,Fu S,Zhang Z,Hu B,Zhang X,Lin S,Du H

Wed Sep 07 00:00:00 UTC 2022

PRR11 promotes cell proliferation by regulating PTTG1 through interacting with E2F1 transcription factor in pan-cancer.

Frontiers in molecular biosciences

Zhang H,He Z,Qiu L,Wei J,Gong X,Xian M,Chen Z,Cui Y,Fu S,Zhang Z,Hu B,Zhang X,Lin S,Du H

Wed Sep 07 00:00:00 UTC 2022

KAT2A/E2F1 Promotes Cell Proliferation and Migration via Upregulating the Expression of UBE2C in Pan-Cancer.

Genes

Lin S,Qiu L,Liang K,Zhang H,Xian M,Chen Z,Wei J,Fu S,Gong X,Ding K,Zhang Z,Hu B,Zhang X,Duan Y,Du H

MA1-23202 was used in Western Blotting and Immunocytochemistry to find that the expression of these three genes was significantly higher in more than 10 tumor types compared to normal tissue.

Sat Oct 08 00:00:00 UTC 2022

KAT2A/E2F1 Promotes Cell Proliferation and Migration via Upregulating the Expression of UBE2C in Pan-Cancer.

Genes

Lin S,Qiu L,Liang K,Zhang H,Xian M,Chen Z,Wei J,Fu S,Gong X,Ding K,Zhang Z,Hu B,Zhang X,Duan Y,Du H

MA1-23202 was used in Western Blotting and Immunocytochemistry to find that the expression of these three genes was significantly higher in more than 10 tumor types compared to normal tissue.

Sat Oct 08 00:00:00 UTC 2022

KAT2A/E2F1 Promotes Cell Proliferation and Migration via Upregulating the Expression of UBE2C in Pan-Cancer.

Genes

Lin S,Qiu L,Liang K,Zhang H,Xian M,Chen Z,Wei J,Fu S,Gong X,Ding K,Zhang Z,Hu B,Zhang X,Duan Y,Du H

MA1-23202 was used in Western Blotting and Immunocytochemistry to find that the expression of these three genes was significantly higher in more than 10 tumor types compared to normal tissue.

Sat Oct 08 00:00:00 UTC 2022

生物信息学

蛋白别名: E2F-1; PBR3; PRB-binding protein E2F-1; RBAP-1; RBBP-3; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; Transcription factor E2F1

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基因别名: E2F-1; E2F1; mKIAA4009; RBAP1; RBBP3; RBP3

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UniProt ID: (Human) Q01094, (Mouse) Q61501, (Rat) O09139

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Entrez Gene ID: (Human) 1869, (Mouse) 13555, (Rat) 399489

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功能

core promoter binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding protein kinase binding sequence-specific DNA binding transcription regulatory region DNA binding

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