Invitrogen

E2F2 Polyclonal Antibody

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E2F2 Antibody (PA5-79179) in WB

Western blot analysis of E2F2 in Lane 1: rat lung tissue lysate, Lane 2: rat heart tissue lysate, Lane 3: rat brain tissue lysate, Lane 4: rat kidney tissue lysate, Lane 5: HeLa whole cell lysate, Lane 6: COLO320 whole cell lysate, Lane 7: A549 whole cell lysate, Lane 8: MCF-7 whole cell lysate, Lane 9: SMMC whole cell lysate using 40-50 µg per well. Sample was incubated with E2F2 (Product # PA5-79179) at a dilution of 0.5 µg/mL.

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产品信息

PA5-79179

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence at the C-terminus of human E2F2 (422-427aa ISDLFDSYDLGDLLIN).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide, 0.05mg thimerosal

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2746295

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

E2F2 is a member of the E2Ffamily of transcription factors. The E2F family plays a crucialrole in the control of cell cycle and action of tumor suppressorproteins and is also a target of the transforming proteins of smallDNA tumor viruses. The E2F proteins contain several evolutionallyconserved domains found in most members of the family. Thesedomains include a DNA binding domain, a dimerization domain whichdetermines interaction with the differentiation regulatedtranscription factor proteins (DP), a transactivation domainenriched in acidic amino acids, and a tumor suppressor proteinassociation domain which is embedded within the transactivationdomain. This protein and another 2 members, E2F1 and E2F3, have anadditional cyclin binding domain. This protein binds specificallyto retinoblastoma protein pRB in a cell-cycle dependent manner, andit exhibits overall 46% amino acid identity to E2F1.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。