Invitrogen

E2F2 Recombinant Rabbit Monoclonal Antibody (23GB5250)

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E2F2 Antibody (MA5-52822) in WB

Western Blot analysis of E2F2. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with E2F2 recombinant monoclonal antibody (Product # MA5-52822) with a dilution of 1:5,000, followed by HRP-conjugated goat anti-rabbit secondary antibody with a dilution of 1:20,000. Image was developed using FeQTM ECL Substrate Kit.

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产品信息

MA5-52822

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:5,000

流式细胞分析 (Flow)

1:2,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293F

分类

Recombinant Monoclonal

类型

Antibody

克隆号

23GB5250

抗原

A synthesized peptide derived from human E2F2 (313-375AA).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice (domestic); Dry ice (international)

靶标信息

E2F2 is a member of the E2Ffamily of transcription factors. The E2F family plays a crucialrole in the control of cell cycle and action of tumor suppressorproteins and is also a target of the transforming proteins of smallDNA tumor viruses. The E2F proteins contain several evolutionallyconserved domains found in most members of the family. Thesedomains include a DNA binding domain, a dimerization domain whichdetermines interaction with the differentiation regulatedtranscription factor proteins (DP), a transactivation domainenriched in acidic amino acids, and a tumor suppressor proteinassociation domain which is embedded within the transactivationdomain. This protein and another 2 members, E2F1 and E2F3, have anadditional cyclin binding domain. This protein binds specificallyto retinoblastoma protein pRB in a cell-cycle dependent manner, andit exhibits overall 46% amino acid identity to E2F1.

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