Invitrogen

EBP50 Polyclonal Antibody

EBP50 Antibody in Immunocytochemistry (ICC/IF)

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EBP50 Antibody (PA1-090) in ICC/IF

Immunofluorescent analysis of EBP50 was performed using 70% confluent log phase T-47D cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with EBP50 Rabbit Polyclonal Antibody (Product # PA1-090) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2... View More

EBP50 Antibody in Immunohistochemistry (IHC)

EBP50 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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EBP50 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of EBP50 was observed in MCF-7, SK-BR-3 and T-47D in higher levels in comparison to MDA-MB-231 in low levels using Anti-EBP50 Polyclonal Antibody (Product # PA1-090 ) in Western Blot. (doi:https://doi.org/10.3892/or.2017.5649). {RE}

产品信息

PA1-090

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.2-1 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

10 µg/mL

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免疫细胞化学 (ICC/IF)

2 µg/mL

查看4篇已发表文章 4篇已发表文章

免疫沉淀 (IP)

Assay-dependent

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产品规格

种属反应

Human, Mouse

已发表种属

Dog, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Bacterially expressed, His-tagged human EBP50.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2191493

产品详细信息

PA1-090 detects ezrin-radixin-moesin (ERM) binding phosphoprotein of 50 kDa (EBP50) from mouse tissues, human T84 and Calu3 cells as well as recombinant human protein.

PA1-090 has been successfully used in Western blot, Immunohistochemistry, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~50 kDa protein representing EBP50 from T84 cell extract. Immunohistochemical staining of EBP50 in mouse airway results in staining of the epithelium.

The PA1-090 immunogen is recombinant human His-tagged EBP50 obtained from bacteria. All His-tag specific antibody has been removed. This product was epitope affinity purified using a synthetic peptide to residues C R(286) S A S S D T S E E L N S Q D S P(302) of human EBP50.

靶标信息

NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Monitoring Effects of Membrane Traffic Via Changes in Cell Polarity and Morphogenesis in Three-Dimensional Human Pluripotent Stem Cell Cysts.

Methods in molecular biology (Clifton, N.J.)

Hamed MM,Taniguchi K,Duncan MC

PA1-090 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to generate hPSC-cysts, a 3-D hPSC-based model of human epiblast lumen formation, which provides unique opportunities to examine the role of membrane traffic during epithelial morphogenesis.

Thu Dec 15 00:00:00 UTC 2022

SARS-CoV-2 replication in airway epithelia requires motile cilia and microvillar reprogramming.

Cell

Wu CT,Lidsky PV,Xiao Y,Cheng R,Lee IT,Nakayama T,Jiang S,He W,Demeter J,Knight MG,Turn RE,Rojas-Hernandez LS,Ye C,Chiem K,Shon J,Martinez-Sobrido L,Bertozzi CR,Nolan GP,Nayak JV,Milla C,Andino R,Jackson PK

PA1-090 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to suggest that motile cilia, microvilli, and mucociliary-dependent mucus flow are critical for efficient virus replication in nasal epithelia.

Thu Jan 05 00:00:00 UTC 2023

βA3/A1-crystallin regulates apical polarity and EGFR endocytosis in retinal pigmented epithelial cells.

Communications biology

Shang P,Stepicheva N,Teel K,McCauley A,Fitting CS,Hose S,Grebe R,Yazdankhah M,Ghosh S,Liu H,Strizhakova A,Weiss J,Bhutto IA,Lutty GA,Jayagopal A,Qian J,Sahel JA,Samuel Zigler J,Handa JT,Sergeev Y,Rajala RVS,Watkins S,Sinha D

PA1-090 was used in Immunohistochemistry to propose that βA3/A1-crystallin acquired its RPE function before evolving as a structural element in the lens, and that in the RPE, it modulates the PI(4,5)P2 pool through PITPβ/PLC signaling axis, coordinates EGFR activation, regulates ezrin phosphorylation and ultimately the cell polarity.

Thu Jul 08 00:00:00 UTC 2021

βA3/A1-crystallin regulates apical polarity and EGFR endocytosis in retinal pigmented epithelial cells.

Communications biology

Thu Jul 08 00:00:00 UTC 2021

βA3/A1-crystallin regulates apical polarity and EGFR endocytosis in retinal pigmented epithelial cells.

Communications biology

Thu Jul 08 00:00:00 UTC 2021

Loss-of-function of EBP50 is a new cause of hereditary peripheral neuropathy: EBP50 functions in peripheral nerve system.

Glia

Song GJ,Gupta DP,Rahman MH,Park HT,Al Ghouleh I,Bisello A,Lee MG,Park JY,Park HH,Jun JH,Chung KW,Choi BO,Suk K

PA1-090 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to show for the first time that loss of function due to EBP50 gene deficiency or mutation can cause peripheral neuropathy.

Tue Sep 01 00:00:00 UTC 2020

Ezrin-Radixin-Moesin Binding Phosphoprotein 50: A Potential Novel Biomarker in Human Papilloma Virus-Associated Head and Neck Squamous Cell Carcinomas.

Head and neck pathology

Shankar A,Crouch DH,Macluskey M

PA1-090 was used in Immunohistochemistry to evaulate the use of EBP50 as a biomarker of High Risk Papilloma Virus infection in head and neck squamous cell carcinoma.

Sat Jun 01 00:00:00 UTC 2019

Establishment of three novel cell lines derived from African American patients with colorectal carcinoma: A unique tool for assessing racial health disparity.

International journal of oncology

Paredes J,Ji P,Lacomb JF,Shroyer KR,Martello LA,Williams JL

Published figure using EBP50 polyclonal antibody (Product # PA1-090) in Western Blot

Mon Oct 01 00:00:00 UTC 2018

NHERF1 and tumor microenvironment: a new scene in invasive breast carcinoma.

Journal of experimental & clinical cancer research : CR

Saponaro C,Vagheggini A,Scarpi E,Centonze M,Catacchio I,Popescu O,Pastena MI,Giotta F,Silvestris N,Mangia A

Published figure using EBP50 polyclonal antibody (Product # PA1-090) in Immunohistochemistry (Paraffin)

Wed May 02 00:00:00 UTC 2018

NHERF1 and tumor microenvironment: a new scene in invasive breast carcinoma.

Journal of experimental & clinical cancer research : CR

Saponaro C,Vagheggini A,Scarpi E,Centonze M,Catacchio I,Popescu O,Pastena MI,Giotta F,Silvestris N,Mangia A

Published figure using EBP50 polyclonal antibody (Product # PA1-090) in Immunohistochemistry (Paraffin)

Wed May 02 00:00:00 UTC 2018

Hierarchical clustering analysis identifies metastatic colorectal cancers patients with more aggressive phenotype.

Oncotarget

Opinto G,Silvestris N,Centonze M,Graziano G,Pinto R,Fucci L,Simone G,Mangia A

Published figure using EBP50 polyclonal antibody (Product # PA1-090) in Immunohistochemistry

Fri Oct 20 00:00:00 UTC 2017

Correctors of mutant CFTR enhance subcortical cAMP-PKA signaling through modulating ezrin phosphorylation and cytoskeleton organization.

Journal of cell science

Abbattiscianni AC,Favia M,Mancini MT,Cardone RA,Guerra L,Monterisi S,Castellani S,Laselva O,Di Sole F,Conese M,Zaccolo M,Casavola V

PA1-090 was used in Immunoprecipitation to demonstrate that the phosphorylation of ezrin together with its binding to phosphatidylinositol-4,5-bisphosphate tethers the F508del cystic fibrosis transmembrane regulator to the actin cytoskeleton, rescuing the sub-membrane compartmentalisation of cAMP and activated PKA.

Tue Mar 15 00:00:00 UTC 2016

Acta histochemica

Demacopulo B,Lema BE,Cabrini RL,Kreimann EL

PA1090 was used in immunohistochemistry to examine the distribution of NHERF1 in ovarian cancer cells

Sat Oct 01 00:00:00 UTC 2016

β-catenin interaction with NHERF1 and RASSF1A methylation in metastatic colorectal cancer patients.

Oncotarget

Schirosi L,Mazzotta A,Opinto G,Pinto R,Graziano G,Tommasi S,Fucci L,Simone G,Mangia A

PA1090 was used in immunohistochemistry - paraffin section to correlate the subcellular expression of beta-catenin, NHERF1, and RASSF1A with the progression of colorectal cancer

Tue Oct 18 00:00:00 UTC 2016

NHERF1/EBP50 Suppresses Wnt-β-Catenin Pathway-Driven Intestinal Neoplasia.

Neoplasia (New York, N.Y.)

Georgescu MM,Gagea M,Cote G

Published figure using EBP50 polyclonal antibody (Product # PA1-090) in Immunofluorescence

Mon Aug 01 00:00:00 UTC 2016

The potential predictive role of nuclear NHERF1 expression in advanced gastric cancer patients treated with epirubicin/oxaliplatin/capecitabine first line chemotherapy.

Cancer biology & therapy

Mangia A,Caldarola L,Dell'Endice S,Scarpi E,Saragoni L,Monti M,Santini D,Brunetti O,Simone G,Silvestris N

Published figure using EBP50 polyclonal antibody (Product # PA1-090) in Immunohistochemistry

Thu May 12 00:00:00 UTC 2016

Fine Needle Aspiration Cytology: A Tool to Study NHERF1 Expression as a Potential Marker of Aggressiveness in Lung Cancer.

Molecular biotechnology

Mangia A,Partipilo G,Schirosi L,Saponaro C,Galetta D,Catino A,Scattone A,Simone G

PA1-090 was used in immunohistochemistry - paraffin section to assess the role of NHERF1 in lung tumors using fine needle aspirates

Mon Jun 01 00:00:00 UTC 2015

Immunoprofile from tissue microarrays to stratify familial breast cancer patients.

Oncotarget

Schirosi L,De Summa S,Tommasi S,Paradiso A,Sambiasi D,Popescu O,Simone G,Mangia A

PA1-090 was used in immunohistochemistry - paraffin section to classify familial breast cancer patients and their immunoprofile from tissue microarrays

Tue Sep 29 00:00:00 UTC 2015

High density of tryptase-positive mast cells in human colorectal cancer: a poor prognostic factor related to protease-activated receptor 2 expression.

Journal of cellular and molecular medicine

Malfettone A,Silvestris N,Saponaro C,Ranieri G,Russo A,Caruso S,Popescu O,Simone G,Paradiso A,Mangia A

PA1-090 was used in immunohistochemistry to study the prognostic significance of a high density of tryptase-positive mast cells and PAR-2 expression levels in colorectal cancer patients

Thu Aug 01 00:00:00 UTC 2013

Characterization of CFTR High Expresser cells in the intestine.

American journal of physiology. Gastrointestinal and liver physiology

Jakab RL,Collaco AM,Ameen NA

PA1-090 was used in immunohistochemistry to study the function of CFTR High Expresser cells in the intestine of rats

Sun Sep 15 00:00:00 UTC 2013

Nuclear NHERF1 expression as a prognostic marker in breast cancer.

Cell death & disease

Paradiso A,Scarpi E,Malfettone A,Addati T,Giotta F,Simone G,Amadori D,Mangia A

PA1-090 was used in immunohistochemistry to study the prognostic value of nuclear expression of NHERF-1 in breast cancer

Thu Nov 07 00:00:00 UTC 2013

The tight-junction protein claudin-6 induces epithelial differentiation from mouse F9 and embryonic stem cells.

PloS one

Sugimoto K,Ichikawa-Tomikawa N,Satohisa S,Akashi Y,Kanai R,Saito T,Sawada N,Chiba H

PA1-090 was used in immunocytochemistry and western blot to study the role of Cldn6 in epithelial differentiation.

Mon Jun 09 00:00:00 UTC 2014

PTEN, NHERF1 and PHLPP form a tumor suppressor network that is disabled in glioblastoma.

Oncogene

Molina JR,Agarwal NK,Morales FC,Hayashi Y,Aldape KD,Cote G,Georgescu MM

PA1-090 was used in western blot to study the regulation of phosphatidylinositol-3-OH kinase-Akt pathway in glioblastoma

Thu Mar 08 00:00:00 UTC 2012

Overexpression of nuclear NHERF1 in advanced colorectal cancer: association with hypoxic microenvironment and tumor invasive phenotype.

Experimental and molecular pathology

Malfettone A,Silvestris N,Paradiso A,Mattioli E,Simone G,Mangia A

PA1-090 was used in immunohistochemistry to study the role of NHERF1 and its potential utility as a biomarker of advanced colorectal cancer

Fri Jun 01 00:00:00 UTC 2012

Involvement of nuclear NHERF1 in colorectal cancer progression.

Oncology reports

Mangia A,Saponaro C,Malfettone A,Bisceglie D,Bellizzi A,Asselti M,Popescu O,Reshkin SJ,Paradiso A,Simone G

PA1-090 was used in immunohistochemistry to study the role of nuclear NHERF1 in the progression of colorectal cancer

Sat Sep 01 00:00:00 UTC 2012

Ezrin-anchored protein kinase A coordinates phosphorylation-dependent disassembly of a NHERF1 ternary complex to regulate hormone-sensitive phosphate transport.

The Journal of biological chemistry

Wang B,Means CK,Yang Y,Mamonova T,Bisello A,Altschuler DL,Scott JD,Friedman PA

Published figure using EBP50 polyclonal antibody (Product # PA1-090) in Western Blot

Fri Jul 13 00:00:00 UTC 2012

Peritumoral vascular invasion and NHERF1 expression define an immunophenotype of grade 2 invasive breast cancer associated with poor prognosis.

BMC cancer

Malfettone A,Saponaro C,Paradiso A,Simone G,Mangia A

PA1-090 was used in immunohistochemistry to study a category of grade 2 breast cancer defined by peritumoral vascular invasion and the expression of NHERF1

Thu Mar 22 00:00:00 UTC 2012

Na+/H+ exchanger regulatory factor 1 (NHERF1) directly regulates osteogenesis.

The Journal of biological chemistry

Liu L,Alonso V,Guo L,Tourkova I,Henderson SE,Almarza AJ,Friedman PA,Blair HC

PA1-090 was used in immunocytochemistry to study the expression of NHERF1 by mineralizing osteoblasts and its role in osteogenesis

Fri Dec 21 00:00:00 UTC 2012

A hierarchy of signals regulates entry of membrane proteins into the ciliary membrane domain in epithelial cells.

The Journal of cell biology

Francis SS,Sfakianos J,Lo B,Mellman I

PA1-090 was used in western blot to investigate the mechanism for the exclusion of podocalyxin from the ciliary membrane domain

Mon Apr 04 00:00:00 UTC 2011

Na+/H+ exchanger regulatory factor 1 expression levels in blood and tissue predict breast tumour clinical behaviour.

Histopathology

Bellizzi A,Mangia A,Malfettone A,Cardone RA,Simone G,Reshkin SJ,Paradiso A

PA1-090 was used in immunohistochemistry to study the prognostic value of sodium/hydrogen exchanger regulatory factor 1 for breast tumour

Wed Jun 01 00:00:00 UTC 2011

Na+/H+ exchanger regulatory factor 1 expression levels in blood and tissue predict breast tumour clinical behaviour.

Histopathology

Bellizzi A,Mangia A,Malfettone A,Cardone RA,Simone G,Reshkin SJ,Paradiso A

PA1-090 was used in immunohistochemistry to study the prognostic value of sodium/hydrogen exchanger regulatory factor 1 for breast tumour

Wed Jun 01 00:00:00 UTC 2011

Na+/H+ exchanger regulatory factor 1 expression levels in blood and tissue predict breast tumour clinical behaviour.

Histopathology

Bellizzi A,Mangia A,Malfettone A,Cardone RA,Simone G,Reshkin SJ,Paradiso A

PA1-090 was used in immunohistochemistry to study the prognostic value of sodium/hydrogen exchanger regulatory factor 1 for breast tumour

Wed Jun 01 00:00:00 UTC 2011

Human epidermal growth factor receptor 2, Na+/H+ exchanger regulatory factor 1, and breast cancer susceptibility gene-1 as new biomarkers for familial breast cancers.

Human pathology

Mangia A,Malfettone A,Saponaro C,Tommasi S,Simone G,Paradiso A

PA1-090 was used in immunohistochemistry to investigate the changes of gene expression in familial breast cancers

Tue Nov 01 00:00:00 UTC 2011

Quantitative protein and mRNA profiling shows selective post-transcriptional control of protein expression by vasopressin in kidney cells.

Molecular & cellular proteomics : MCP

Khositseth S,Pisitkun T,Slentz DH,Wang G,Hoffert JD,Knepper MA,Yu MJ

PA1-090 was used in western blot to study the post-transcriptional control of protein expression by vasopressin in kidney cells

Sat Jan 01 00:00:00 UTC 2011

Na/H exchanger regulatory factors control parathyroid hormone receptor signaling by facilitating differential activation of G(alpha) protein subunits.

The Journal of biological chemistry

Wang B,Ardura JA,Romero G,Yang Y,Hall RA,Friedman PA

PA1-090 was used in western blot to investigate the mechanism of parathyroid hormone receptor pathway modulation mediated by Na/H exchanger regulatory factors

Fri Aug 27 00:00:00 UTC 2010

Biological role of NHERF1 protein expression in breast cancer.

Histopathology

Mangia A,Chiriatti A,Bellizzi A,Malfettone A,Stea B,Zito FA,Reshkin SJ,Simone G,Paradiso A

PA1-090 was used in immunohistochemistry to investigate the role of sodium/hydrogen exchanger regulatory factor in tumorigenesis

Sun Nov 01 00:00:00 UTC 2009

Hepatocyte nuclear factor 4alpha, a key factor for homeostasis, cell architecture, and barrier function of the adult intestinal epithelium.

Molecular and cellular biology

Cattin AL,Le Beyec J,Barreau F,Saint-Just S,Houllier A,Gonzalez FJ,Robine S,Pinçon-Raymond M,Cardot P,Lacasa M,Ribeiro A

PA1-090 was used in western blot to study the role of hepatocyte nuclear factor 4 in the adult mouse small intestine.

Tue Dec 01 00:00:00 UTC 2009

Expression and clinicopathological significance of oestrogen-responsive ezrin-radixin-moesin-binding phosphoprotein 50 in breast cancer.

Histopathology

Song J,Bai J,Yang W,Gabrielson EW,Chan DW,Zhang Z

PA1-090 was used in immunohistochemistry to study the expression of ezrin-radixin-moesin-binding phosphoprotein 50 in breast cancer and its clinical relevance

Sun Jul 01 00:00:00 UTC 2007

Expression and clinicopathological significance of oestrogen-responsive ezrin-radixin-moesin-binding phosphoprotein 50 in breast cancer.

Histopathology

Song J,Bai J,Yang W,Gabrielson EW,Chan DW,Zhang Z

PA1-090 was used in immunohistochemistry to study the expression of ezrin-radixin-moesin-binding phosphoprotein 50 in breast cancer and its clinical relevance

Sun Jul 01 00:00:00 UTC 2007

Proteomic analysis of peripheral blood mononuclear cells: selective protein processing observed in patients with rheumatoid arthritis.

Journal of proteome research

Schulz M,Dotzlaw H,Mikkat S,Eggert M,Neeck G

PA1-090 was used in western blot to investigate the changes of proteins in peripheral blood mononuclear cells from patients with rheumatoid arthritis

Sat Sep 01 00:00:00 UTC 2007

Expression of ion transport-associated proteins in human efferent and epididymal ducts.

Reproduction (Cambridge, England)

Kujala M,Hihnala S,Tienari J,Kaunisto K,Hästbacka J,Holmberg C,Kere J,Höglund P

PA1-090 was used in immunohistochemistry to investigate the expression and localization of ion transport-associated proteins in epididymal and efferent ducts

Sun Apr 01 00:00:00 UTC 2007

Receptor activity-modifying protein (RAMP) isoform-specific regulation of adrenomedullin receptor trafficking by NHERF-1.

The Journal of biological chemistry

Bomberger JM,Spielman WS,Hall CS,Weinman EJ,Parameswaran N

PA1-090 was used in immunocytochemistry to demonstrate the role of NHERF-1 and RAMP3 on receptor internalization.

Fri Jun 24 00:00:00 UTC 2005

Foxj1 is required for apical localization of ezrin in airway epithelial cells.

Journal of cell science

Huang T,You Y,Spoor MS,Richer EJ,Kudva VV,Paige RC,Seiler MP,Liebler JM,Zabner J,Plopper CG,Brody SL

PA1-090 was used in immunocytochemistry to investigate the role of Foxj1 in the apical localization of ezrin in airway epithelial cells.

Mon Dec 15 00:00:00 UTC 2003

Activation-independent parathyroid hormone receptor internalization is regulated by NHERF1 (EBP50).

The Journal of biological chemistry

Sneddon WB,Syme CA,Bisello A,Magyar CE,Rochdi MD,Parent JL,Weinman EJ,Abou-Samra AB,Friedman PA

PA1-090 was used in western blot to study the effect of NHERF1 on the conditional efficacy of PTH fragments.

Fri Oct 31 00:00:00 UTC 2003

生物信息学

蛋白别名: EBP50; ezrin-radixin-moesin binding phosphoprotein-50; Ezrin-radixin-moesin-binding phosphoprotein 50; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na+/H+ exchange regulatory co-factor; NHERF-1; Regulatory cofactor of Na(+)/H(+) exchanger; So; Sodium-hydrogen exchanger regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1; Solute carrier family 9 isoform A3 regulatory factor 1; solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1

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基因别名: EBP-50; EBP50; NHE-RF; NHERF; NHERF-1; NHERF1; NPHLOP2; SLC9A3R1

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UniProt ID: (Human) O14745, (Mouse) P70441

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Entrez Gene ID: (Human) 9368, (Mouse) 26941

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功能