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ECH1 Antibody (PA5-88881) in IHC (P)
Immunohistochemistry analysis of ECH1 in paraffin-embedded human placenta. Samples were incubated with ECH1 Polyclonal antibody (Product # PA5-88881) using a dilution of 1:150 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Details
PA5-88881
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human ECH1 (NP_0013892)
Conjugate
Form
Liquid
Concentration
0.08 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805192
Product Specific Information
Immunogen sequence: MAAGIVASRR LRDLLTRRLT GSNYPGLSIS LRLTGSSAQE EASGVALGEA PDHSYESLRV TSAQKHVLHV QLNRPNKRNA MNKVFWREMV ECFNKISRDA DCRAVVISGA GKMFTAGIDL MDMASDILQP KGDDVARISW YLRDIITRYQ ETFNVIERCP KPVIAAVHGG CIGGGVDLVT ACDIRYCAQD AFFQVKEVDV GLAADVGTLQ RLPKVIGNQS LVNELAFTAR KMMADEALGS GLVSRVFPDK EVMLDAALAL AAEISSKSPV AVQSTKVNLL YSRDHSVAES LNYVASWNMS MLQTQDLVKS VQATTENKEL KTVTFSKL; Positive Samples: A375, BxPC3, Hep G2, Jurkat, MCF7, K-562, Mouse testis, Rat liver; Cellular Location: Mitochondrion, Peroxisome
Target Information
This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial; delta3,5-delta2,4-dienoyl-CoA isomerase; delta3,5-delta2,4-dienoyl-coenzyme A isomerase; dienoyl-CoA isomerase; enoyl CoA hydratase 1, peroxisomal; enoyl Coenzyme A hydratase 1, peroxisomal; enoyl hydratase-like protein peroxisomal; enoyl hydratase-like protein, peroxisomal; enoyl-CoA hydratase 1, peroxisomal; Peroxisomal enoyl hydratase-like protein; peroxisomal enoyl-CoA hydratase 1; peroxisomal/mitochondrial dienoyl-CoA isomerase
Gene Aliases: AA617331; ECH1; HPXEL; Pxel
UniProt ID: (Human) Q13011, (Rat) Q62651, (Mouse) O35459
Entrez Gene ID: (Human) 1891, (Rat) 64526, (Mouse) 51798
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