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ECM29 Antibody (PA3-035) in ICC/IF
Immunofluorescent analysis of Ecm29 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Ecm29 Rabbit Polyclonal Antibody (Product # PA3-035) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Produc... View More
产品信息
PA3-035
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Human,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to the C-terminal residues R(1821) P E L Q E K A A L L K K T L E N L E(1839) of human Ecm29.
偶联物
形式
Liquid
浓度
Conc. Not Determined
保存液
whole serum
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2130523
产品详细信息
PA3-035 detects Ecm29 in human and mouse samples.
PA3-035 has been used successfully in Western blot and immunofluorescence procedures. In Western blot analysis of HeLa cell extracts this antibody detects a ~205 kDa protein representing Ecm29.
The PA3-035 immunogen is a synthetic peptide corresponding to the C-terminal residues R(1821) P E L Q E K A A L L K K T L E N L E(1839) of human Ecm29.
靶标信息
Ecm29 appears to be one of three proteins which associate significantly with proteasomes. Ecm29 has been shown to enhance the stability of the proteasome. Ecm29 deletion has been shown to produce 26S proteasomes which dissociated in the absence of ATP. It was thus suggested that Ecm29 tethers the regulatory complex to the proteasome.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ecm29; ECM29 homolog, proteasome accessory protein; FLJ22036; homolog of yeast Ecm29; KIAA1962; Proteasome adapter and scaffold protein ECM29; Proteasome-associated protein ECM29 homolog; RP11-386D8.2
基因别名: AW558785; BB181316; ECM29; Ecpas; Kiaa0368; mKIAA0368
UniProt ID: (Mouse) Q6PDI5
Entrez Gene ID: (Mouse) 230249, (Human) 23392
