Invitrogen

ECT2 Polyclonal Antibody

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2 篇参考文献

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抗体检测数据 (2)
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ECT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ECT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ECT2 Antibody (PA5-67612) in IHC (P)

Immunohistochemical analysis of ECT2 in paraffin-embedded Human cervical cancer tissue using (left) ECT2 Polyclonal Antibody (Product # PA5-67612) at a 1:20 dilution and (right) is treated with synthetic peptide. (Original magnification: x200).

ECT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ECT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ECT2 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

ECT2 Antibody in Western Blot (WB)

Product Image Gallery

ECT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of ECT2 in paraffin-embedded Human cervical cancer tissue using (left) ECT2 Polyclonal Antibody (Product # PA5-67612) at a 1:20 dilution and (right) is treated with synthetic peptide. (Original magnification: x200).

ECT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of ECT2 in paraffin-embedded Human tonsil tissue using (left) ECT2 Polyclonal Antibody (Product # PA5-67612) at a 1:20 dilution and (right) is treated with synthetic peptide. (Original magnification: x200).

ECT2 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Fig. 3 Downregulation of ECT2 inhibits the proliferation and migration of HCC cells. A Representative immunohistochemistry stain of ECT2 in HCC and para-cancerous normal tissues. B Quantification of PCR analysis of ECT2 expression in HCC and para-cancerous normal tissues. C Western blot analysis of ECT2 after transfection of ECT2-targeting shRNA. D ECT2 downregulation inhibits cell proliferation. Cancer cells were transfected with ECT2-targeting shRNA, and the absorption (A450 nm) was detected at 0, 24, 48, and 72 h. E Cancer cells transfected with ECT2-targeting shRNA and the normal control were assayed for clonogenicity in adherent cultures. F EdU incorporation assay was used to examine the proliferation of cancer cells after ECT2 knockdown. G Transwell assay was used for cancer cells transfected with ECT2-targeting shRNA (magnification 100x). H Wound-healing assay was used for cancer cells transfected with ECT2-targeting shRNA (magnification 50x, scale bar: 500 mum). Error bars represent means +- SD. * p < 0.05, ** p < 0.01. NS means ""not significant"" by paired two-sided Student's t -test.

ECT2 Antibody in Western Blot (WB) — Fig. 6 ECT2 overexpression increases the production of lactic acid and subsequently promotes M2 macrophage polarization. A Correlation analysis between ECT2 and macrophages. B Western blot analysis of p-AKT, PLK1, and AKT expression levels after ECT2 was overexpressed in the HepG2 cell line. C Lactate acid production by cancer cells was evaluated after ECT2 was overexpressed. D Immunofluorescence analysis of macrophage polarization markers. E Western blot analysis was used to evaluate the effect of LDHA knockdown. F Lactate acid production by cancer cells was evaluated after ECT2 was overexpressed and/or LDHA was knocked down. G CD206 and CD163, which are polarization markers, were evaluated by flow cytometry after macrophages were co-cultured with cancer cells whose LDHA and/or ECT2 expression was influenced. Error bars represent means +- SD. * p < 0.05, ** p < 0.01. NS means ""not significant"" by paired two-sided Student's t -test.

产品信息

PA5-67612

应用

建议稀释比

已发表文章

免疫印迹 (WB)

-

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免疫组化 (IHC)

-

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免疫组化（石蜡） (IHC (P))

1:20-1:100

-

产品规格

种属反应

Human, Mouse

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthesized peptide derived from human ECT2.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

affinity chromatography

保存液

PBS, pH 7.4, with 40% glycerol

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2688413

产品详细信息

Predicted to react with Mouse samples.

靶标信息

The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

ECT2 overexpression promotes the polarization of tumor-associated macrophages in hepatocellular carcinoma via the ECT2/PLK1/PTEN pathway.

Cell death & disease

Xu D,Wang Y,Wu J,Zhang Z,Chen J,Xie M,Tang R,Chen C,Chen L,Lin S,Luo X,Zheng J

Published figure using ECT2 polyclonal antibody (Product # PA5-67612) in Western Blot

Mon Feb 08 00:00:00 UTC 2021

ECT2 overexpression promotes the polarization of tumor-associated macrophages in hepatocellular carcinoma via the ECT2/PLK1/PTEN pathway.

Cell death & disease

Xu D,Wang Y,Wu J,Zhang Z,Chen J,Xie M,Tang R,Chen C,Chen L,Lin S,Luo X,Zheng J

Published figure using ECT2 polyclonal antibody (Product # PA5-67612) in Western Blot

Mon Feb 08 00:00:00 UTC 2021

生物信息学

蛋白别名: epithelial cell transforming sequence 2 oncogene; Epithelial cell-transforming sequence 2 oncogene; FLJ10461; MGC138291; Protein ECT2

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基因别名: AI528536; ARHGEF31; ECT2; mKIAA4037

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UniProt ID: (Human) Q9H8V3, (Mouse) Q07139

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Entrez Gene ID: (Human) 1894, (Mouse) 13605

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signal transducer activity guanyl-nucleotide exchange factor activity Rho guanyl-nucleotide exchange factor activity GTPase activator activity protein binding Rho GTPase binding protein homodimerization activity G-protein modulator protein-binding activity modulator guanyl-nucleotide exchange factor

cell morphogenesis cytokinesis protein transport activation of protein kinase activity positive regulation of cytokinesis regulation of Rho protein signal transduction intracellular signal transduction positive regulation of protein import into nucleus positive regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of GTPase activity positive regulation of neuron differentiation regulation of protein kinase activity regulation of small GTPase mediated signal transduction protein homooligomerization regulation of attachment of spindle microtubules to kinetochore cellular response to hydrogen peroxide bicellular tight junction assembly cellular response to calcium ion cellular response to ionizing radiation activation of GTPase activity transport cell cycle signal transduction nervous system development cell differentiation cell division