Invitrogen

EDC4 Polyclonal Antibody

EDC4 Antibody in Immunocytochemistry (ICC/IF)

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EDC4 Antibody (PA5-30485) in ICC/IF

Immunofluorescent analysis of EDC4 (green) in Hela cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 2% BSA in PBS for 60 minutes at room temperature. Cells were stained with a EDC4 polyclonal antibody (Product # PA5-30485) at a dilution of 2 µg/mL in staining buffer for 3 hour at room temperature, and then incubated with a Goat anti-rabbit IgG Secondary Antibody, Alexa Fluor 488 conjugate (Product # A32731) at a dilution of 1:1000 for 1 hour a... View More

EDC4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-30485

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 903 and 1181 of Human EDC4

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2547959

产品详细信息

Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7, mouse brain.

Predicted reactivity: Mouse (96%), Rat (97%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

In the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro).

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。