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EEF1A2 Polyclonal Antibody

EEF1A2 Antibody in Immunocytochemistry (ICC/IF)

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EEF1A2 Antibody (600-101-ML6) in ICC/IF

Immunofluorescence of Goat Anti-EEF1A2 Antibody. Cell Line: MCF-7 cells. Fixative: 100% MeOH. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-EEF1A2 at 15µg/mL overnight at 2-8°C. Secondary Antibody: Donkey Anti-Goat DyLight™488 Antibody (p/n 605-741-125) at 5µg/mL for 1 hr at RT. Nuclear Counterstain: DAPI. Staining: (A) DAPI. (B) Anti-EEF1A2 + secondary. (C) merge A+B. (D). Secondary Only. Localization Expected: nucleoli, cytosol.

EEF1A2 Antibody in Immunohistochemistry (IHC)

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产品信息

600-101-ML6

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化 (IHC)

1:200

免疫细胞化学 (ICC/IF)

15 µg/mL

酶联免疫吸附实验 (ELISA)

1:10,000-1:50,000

产品规格

种属反应

Human, Mouse

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Anti-EEF1A2 antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a near C-terminal portion of human EEF1A2 conjugated to Keyhole Limpet Hemocyanin (KLH).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.77 mg/mL

纯化类型

Affinity chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against human EEF1A2. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to human, mouse, rat, bovine, and rabbit.

靶标信息

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer. [provided by RefSeq, Mar 2014]

仅用于科研。不用于诊断过程。未经明确授权不得转售。