Invitrogen

EEF2 Recombinant Rabbit Monoclonal Antibody (23GB1550)

EEF2 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 4

EEF2 Antibody (MA5-53966) in ICC/IF

Immunocytochemistry analysis of EEF2 in HepG2 cells. Nuclei were stained blue with DAPI; EEF2 recombinant monoclonal antibody (Product # MA5-53966) with a dilution of 1:1,000 was stained magenta with Alexa Fluor 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Product Image Gallery

EEF2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with Eukaryotic translation elongation factor 2 shRNA and decrease in signal intensity was observed in Western Blotting using Anti-EEF2 Recombinant Rabbit Monoclonal Antibody (23GB1550) (Product # MA5-53966) at a dilution of 1:5,000. Secondary antibody used was a goat anti-rabbit (HRP) at a dilution of 1:20,000. {KD}

产品信息

MA5-53966

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:5,000

免疫细胞化学 (ICC/IF)

1:1,000

流式细胞分析 (Flow)

1:2,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293F

分类

Recombinant Monoclonal

类型

Antibody

克隆号

23GB1550

抗原

A synthesized peptide derived from human EEF2 (800-858AA).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice (domestic); Dry ice (international)

靶标信息

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。