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Invitrogen
EEF2K Recombinant Rabbit Monoclonal Antibody (14H7L26)
FIGURE: 1 / 3
EEF2K Antibody (702858) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1), Hep G2 (Lane 2), HCT 116 (Lane 3), A-431 (Lane 4), A549 (Lane 5) and COS-7 (Lane 6). The blot was probed with Anti-EEF2K Recombinant Rabbit Monoclonal Antibody (Product # 702858, 1:5000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:5000 dilution). A ~105 kDa band corresponding to EEF2K was observed across the cell lines tested.
Product Details
702858
Product Specifications
Species Reactivity
Human,
Non-human primate
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
14H7L26
Immunogen
Protein corresponding to human EEF2K [aa627-aa725]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2762420
Product Specific Information
This antibody is predicted to react with Monkey, Cat, Horse.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
EEF2K is a highly conserved Serine/Threoning kinase in the calmodulin signaling pathway whose activity is increased in cancers including breast cancer. EEF2K (eukaryotic elongation factor 2 kinase) is a threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Up activation, by a variety of upstream kinases including AMPK or TRPM7, EEF2K phosphorylates the elongation factor EEF2 at a single site. Then, EEF2K renders EEF2 unable to bind ribosomes and inactive - in turn, the rate of protein synthesis is reduced.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase; calmodulin-dependent protein kinase III; EC 2.7.11.20; eEF-2 kinase; EF2K; elongation factor-2 kinase; euk; eukaroytic elongation factor 2 kinase; Eukaryotic elongation factor 2 kinase; eukaryotic elongation factor-2 kinase; kinase eEF2K
Gene Aliases: eEF-2K; EEF2K; HSU93850
UniProt ID: (Human) O00418
Entrez Gene ID: (Human) 29904
response to ischemia
translational elongation
regulation of protein autophosphorylation
cellular response to insulin stimulus
negative regulation of apoptotic process
positive regulation of endocytosis
protein autophosphorylation
positive regulation of synapse assembly
positive regulation of dendritic spine morphogenesis
cellular response to calcium ion
cellular response to cAMP
cellular response to anoxia
cellular response to brain-derived neurotrophic factor stimulus
response to prolactin
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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