Invitrogen

EGF Polyclonal Antibody, PeproTech®

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EGF Antibody (500-P174G-1MG) in WB

Western Blot: To detect Murine EGF by Western Blot analysis EGF Polyclonal Antibody (Product # 500-P174G-1MG) can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine EGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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产品信息

500-P174G-1MG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

酶联免疫吸附实验 (ELISA)

0.5-2.0 µg/mL

产品规格

种属反应

Mouse

宿主/亚型

Goat

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Murine EGF

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

AB_2929636

产品详细信息

AA Sequence of recombinant protein: NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR.

Preparation: Produced from sera of goats immunized with highly pure Recombinant Murine EGF. Anti-Murine EGF-specific antibody was purified by affinity chromatography employing an immobilized Murine EGF matrix.

Sandwich ELISA: To detect Murine EGF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine EGF (500-P174GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine EGF.

Western Blot: To detect Murine EGF by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine EGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

靶标信息

EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.

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