Invitrogen

EGF Polyclonal Antibody

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EGF Antibody (PA5-96195) in WB

Western blot analysis of EGF in extracts of 293T cells. Samples were incubated with EGF Polyclonal antibody (Product # PA5-96195) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

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产品信息

PA5-96195

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

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酶联免疫吸附实验 (ELISA)

1 µg/mL

点杂交 (DB)

1:500-1:2,000

产品规格

种属反应

Human, Mouse

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 971-1023 of human EGF (NP_0019542)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.29 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2807997

产品详细信息

Positive Samples: Mouse kidney; Cellular Location: Membrane, Single-pass type I membrane protein

Immunogen sequence: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

靶标信息

EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Mice lacking MBNL1 and MBNL2 exhibit sudden cardiac death and molecular signatures recapitulating myotonic dystrophy.

Human molecular genetics

Lee KY,Seah C,Li C,Chen YF,Chen CY,Wu CI,Liao PC,Shyu YC,Olafson HR,McKee KK,Wang ET,Yeh CH,Wang CH

PA5-96195 was used in Western Blotting to demonstrate that complete ablation of MBNL1/2 in cardiomyocytes is essential for generating sudden death due to lethal cardiac rhythms and reveal potential mechanisms for DM heart pathogenesis.