Invitrogen

EGLN1/EGLN2 Polyclonal Antibody

EGLN1/EGLN2 Antibody in Western Blot (WB)

图: 1 / 1

EGLN1/EGLN2 Antibody (PA5-109677) in WB

Western blot analysis of EGLN1/EGLN2 in extracts of various cell lines. Samples were incubated with EGLN1/EGLN2 Polyclonal antibody (Product # PA5-109677) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Product Image Gallery

产品信息

PA5-109677

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence within amino acids 250-350 of human EGLN1/EGLN2 (NP_0713341/NP_4442741)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.81 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2855088

产品详细信息

Immunogen sequence: DIRGDKITWI EGKEPGCETI GLLMSSMDDL IRHCNGKLGS YKINGRTKAM VACYPGNGTG YVRHVDNPNG DGRCVTCIYY LNKDWDAKVS GGILRIFPEG K

靶标信息

Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. Target proteins are preferentially recognized via a LXXLAP motif.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: Egl nine homolog 1; Egl nine homolog 2; egl nine-like protein 1; egl-9 family hypoxia-inducible factor 1; egl-9 family hypoxia-inducible factor 2; EIT-6; Estrogen-induced tag 6; HIF prolyl hydroxylase 2; HIF-PH1; HIF-PH2; HIF-prolyl hydroxylase 1; HIF-prolyl hydroxylase 2; HPH-3; Hypoxia-inducible factor prolyl hydroxylase 1; Hypoxia-inducible factor prolyl hydroxylase 2; PHD1; PHD2; Prolyl hydroxylase domain-containing protein 1; Prolyl hydroxylase domain-containing protein 2; Prolyl hydroxylase EGLN2; SM-20; zinc finger MYND domain-containing protein 6

查看更多收起

基因别名: C1orf12; ECYT3; EGLN1; EGLN2; EIT6; HALAH; HIF-PH1; HIF-PH2; HIFPH1; HIFPH2; HPH-1; HPH-2; HPH-3; HPH2; PHD1; PHD2; PNAS-118; PNAS-137; SM20; ZMYND6

查看更多收起

UniProt ID: (Human) Q9GZT9, (Human) Q96KS0

查看更多收起

Entrez Gene ID: (Human) 54583, (Human) 112398

查看更多收起

功能

iron ion binding protein binding oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors enzyme binding L-ascorbic acid binding peptidyl-proline dioxygenase activity peptidyl-proline 4-dioxygenase activity ferrous iron binding oxygen sensor activity

参与通路