Invitrogen

EGR2 Monoclonal Antibody (erongr2), PE, eBioscience™

图: 1 / 12

EGR2 Antibody (12-6691-82) in Flow

C57Bl/6 splenocytes stimulated overnight with plate-bound Anti-Mouse CD3e Functional Grade Purified (Product # 16-0031-82) were surface stained with Anti-Mouse CD19 PerCP-Cy5-5 (Product # 45-0193-82). Cells were then fixed and permeabilized using the Foxp3 Staining Buffer Set (Product # 00-5523-00) and stained intracellularly with 0.125 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (left) or 0.125 µg of Anti-Mouse Egr2 PE (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

产品信息

12-6691-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

查看17篇已发表文章 17篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

erongr2

抗原

E. coli-derived protein, aa 1-319

查看免疫原

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10717804

产品详细信息

Description: This erongr2 monoclonal antibody recognizes mouse early growth response 2 (Egr2, Krox-20). Egr2 is a zinc finger-containing transcription factor that is important for the development of T cells and NKT cells. Egr2 is predominantly expressed in the thymus and nervous system, and it is also upregulated in T cells following TCR-crosslinking. Upregulation in all non-adherent mouse splenocytes has been observed following stimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin. Egr2 is believed to antagonize T cell activation and has also been reported to promote apoptosis. The erongr2 antibody also appears to modestly stain a subpopulation of T cells and B cells in freshly isolated spleen cells. The erongr2 monoclonal antibody is specific for Egr2 and does not crossreact to Egr3, based on immunoblot analysis.

Applications Reported: This erongr2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This erongr2 antibody has been tested by intracellular flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The EGR2 gene provides instructions for making a protein called early growth response 2, which is part of the early growth response family of proteins. These proteins bind to specific areas of DNA and help control the activity of particular genes. On the basis of this action, the proteins are referred to as transcription factors. The early growth response 2 protein activates several genes that are involved in the formation and maintenance of myelin, the fatty substance that covers and protects nerve cells. Myelin promotes the efficient transmission of nerve impulses. If myelin is lost (demyelination) or its structure is disrupted, the transmission of nerve impulses is impaired. Mutations in the EGR2 gene can cause two forms of Charcot-Marie-Tooth disease, type 1D or type 4E (sometimes called congenital hypomyelinating neuropathy) or a severe form of type 1D (sometimes called Dejerine-Sottas syndrome) that begins during infancy or early childhood.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

CD11c+ myeloid cell exosomes reduce intestinal inflammation during colitis.

JCI insight

Bauer KM,Nelson MC,Tang WW,Chiaro TR,Brown DG,Ghazaryan A,Lee SH,Weis AM,Hill JH,Klag KA,Tran VB,Thompson JW,Ramstead AG,Monts JK,Marvin JE,Alexander M,Voth WP,Stephens WZ,Ward DM,Petrey AC,Round JL,O'Connell RM

Published figure using EGR2 monoclonal antibody (Product # 12-6691-82) in Flow Cytometry

Mon Oct 10 00:00:00 UTC 2022

Pro-inflammatory immunity supports fibrosis advancement in epidermolysis bullosa: intervention with Ang-(1-7).

EMBO molecular medicine

Bernasconi R,Thriene K,Romero-Fernández E,Gretzmeier C,Kühl T,Maler M,Nauroy P,Kleiser S,Rühl-Muth AC,Stumpe M,Kiritsi D,Martin SF,Hinz B,Bruckner-Tuderman L,Dengjel J,Nyström A

Published figure using EGR2 monoclonal antibody (Product # 12-6691-82) in Flow Cytometry

Thu Oct 07 00:00:00 UTC 2021

Effector T cell responses unleashed by regulatory T cell ablation exacerbate oral squamous cell carcinoma.

Cell reports. Medicine

Chao JL,Korzinkin M,Zhavoronkov A,Ozerov IV,Walker MT,Higgins K,Lingen MW,Izumchenko E,Savage PA

Published figure using EGR2 monoclonal antibody (Product # 12-6691-82) in Flow Cytometry

Tue Sep 21 00:00:00 UTC 2021

CAR-T cell-mediated depletion of immunosuppressive tumor-associated macrophages promotes endogenous antitumor immunity and augments adoptive immunotherapy.

Nature communications

Rodriguez-Garcia A,Lynn RC,Poussin M,Eiva MA,Shaw LC,O'Connor RS,Minutolo NG,Casado-Medrano V,Lopez G,Matsuyama T,Powell DJ

Published figure using EGR2 monoclonal antibody (Product # 12-6691-82) in Flow Cytometry

Tue Feb 09 00:00:00 UTC 2021

Histone deacetylase 3 represses cholesterol efflux during CD4⁺ T-cell activation.

eLife

Wilfahrt D,Philips RL,Lama J,Kizerwetter M,Shapiro MJ,McCue SA,Kennedy MM,Rajcula MJ,Zeng H,Shapiro VS

12-6691-82 was used in Flow cytometry/Cell sorting to demonstrate HDAC3 is essential during CD4+ T-cell activation to repress cholesterol efflux.

Thu Dec 02 00:00:00 UTC 2021

Macrophages Expressing GALC Improve Peripheral Krabbe Disease by a Mechanism Independent of Cross-Correction.

Neuron

Weinstock NI,Shin D,Dhimal N,Hong X,Irons EE,Silvestri NJ,Reed CB,Nguyen D,Sampson O,Cheng YC,Lau JTY,Bongarzone ER,Kofler J,Escolar ML,Gelb MH,Wrabetz L,Feltri ML

12-6691 was used in Flow cytometry/Cell sorting to show that cross-correction does not occur efficiently in vivo and that Galc-deficient Schwann cells autonomously produce psychosine.

Wed Jul 08 00:00:00 UTC 2020

Mycobacterial Trehalose 6,6'-Dimycolate-Induced M1-Type Inflammation.

The American journal of pathology

Nguyen TKT,d'Aigle J,Chinea L,Niaz Z,Hunter RL,Hwang SA,Actor JK

Published figure using EGR2 monoclonal antibody (Product # 12-6691-82) in Flow Cytometry

Sat Feb 01 00:00:00 UTC 2020

African-centric TP53 variant increases iron accumulation and bacterial pathogenesis but improves response to malaria toxin.

Nature communications

Singh KS,Leu JI,Barnoud T,Vonteddu P,Gnanapradeepan K,Lin C,Liu Q,Barton JC,Kossenkov AV,George DL,Murphy ME,Dotiwala F

12-6691-82 was used in Flow Cytometry to show that this ferroptotic defect causes iron accumulation in P47S macrophages.

Fri Jan 24 00:00:00 UTC 2020

Thymic iNKT single cell analyses unmask the common developmental program of mouse innate T cells.

Nature communications

Harsha Krovi S,Zhang J,Michaels-Foster MJ,Brunetti T,Loh L,Scott-Browne J,Gapin L

12-6691 was used in Flow cytometry/Cell sorting to highlight the common requirements for the development of innate-like T cells with a focus on how Hivep3 impacts the maturation of these lymphocytes.

Mon Dec 07 00:00:00 UTC 2020

SHIP-1 Regulates Phagocytosis and M2 Polarization Through the PI3K/Akt-STAT5-Trib1 Circuit in Pseudomonas aeruginosa Infection.

Frontiers in immunology

Qin S,Li J,Zhou C,Privratsky B,Schettler J,Deng X,Xia Z,Zeng Y,Wu H,Wu M

12-6691-82 was used in Flow cytometry/Cell sorting to reveal a previously unrecognized role of SHIP-1 in inflammatory responses and macrophage homeostasis during P. aeruginosa infection through a PI3K/Akt-STAT5-Trib1 axis.

Thu Mar 25 00:00:00 UTC 2021

Transcription factors early growth response gene (Egr) 2 and 3 control inflammatory responses of tolerant T cells.

Immunity, inflammation and disease

Omodho B,Miao T,Symonds ALJ,Singh R,Li S,Wang P

12-6691 was used in Flow cytometry/Cell sorting to show that despite impaired proliferation and IL2 production, tolerant T cells can display inflammatory responses in response to antigen stimulation and this is controlled at least partly by Egr2 and 3.

Fri Jun 01 00:00:00 UTC 2018

Egr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation.

The Journal of experimental medicine

Miao T,Symonds ALJ,Singh R,Symonds JD,Ogbe A,Omodho B,Zhu B,Li S,Wang P

Mon Jun 05 00:00:00 UTC 2017

A shared Runx1-bound Zbtb16 enhancer directs innate and innate-like lymphoid lineage development.

Nature communications

Mao AP,Ishizuka IE,Kasal DN,Mandal M,Bendelac A

Mon Oct 16 00:00:00 UTC 2017

Adverse early life environment increases hippocampal microglia abundance in conjunction with decreased neural stem cells in juvenile mice.

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience

Cohen S,Ke X,Liu Q,Fu Q,Majnik A,Lane R

12-6691 was used in Flow cytometry/Cell sorting to observe that adverse early life environment (AELE) significantly decreased neural stem cell and neuronal numbers in juvenile mice.

Thu Dec 01 00:00:00 UTC 2016

TGF-β3-expressing CD4+CD25(-)LAG3+ regulatory T cells control humoral immune responses.

Nature communications

Okamura T,Sumitomo S,Morita K,Iwasaki Y,Inoue M,Nakachi S,Komai T,Shoda H,Miyazaki J,Fujio K,Yamamoto K

12-6691 was used in Flow cytometry/Cell sorting to investigate the role of GF-β3-expressing CD4+CD25(-)LAG3+ Tregs in humoral immune responses.

Thu Feb 19 00:00:00 UTC 2015

Egr-2 transcription factor is required for Blimp-1-mediated IL-10 production in IL-27-stimulated CD4+ T cells.

European journal of immunology

Iwasaki Y,Fujio K,Okamura T,Yanai A,Sumitomo S,Shoda H,Tamura T,Yoshida H,Charnay P,Yamamoto K

Mon Apr 01 00:00:00 UTC 2013

Elevated and sustained expression of the transcription factors Egr1 and Egr2 controls NKT lineage differentiation in response to TCR signaling.

Nature immunology

Seiler MP,Mathew R,Liszewski MK,Spooner CJ,Barr K,Meng F,Singh H,Bendelac A

Sun Feb 05 00:00:00 UTC 2012

生物信息学

蛋白别名: DKFZp686J1957; E3 SUMO-protein ligase EGR2; E3 SUMO-protein transferase ERG2; Early growth response protein 2; EGR-2; FLJ1454; FLJ14547; Krox-20 homolog; OTTHUMP00000062133; Zinc finger protein Krox-20

查看更多收起

基因别名: Egr-2; Egr2; Krox-20; Krox20; NGF1-B; Zfp-25; Zfp-6

查看更多收起

UniProt ID: (Mouse) P08152

查看更多收起

Entrez Gene ID: (Mouse) 13654

查看更多收起

功能