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EHD1 Antibody (MA5-42814) in IHC (P)
Immunohistochemistry analysis of EHD1 in paraffin-embedded human appendix. Samples were incubated with EHD1 Monoclonal antibody (Product # MA5-42814) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
产品信息
MA5-42814
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Not Applicable
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
5S4P9
抗原
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EHD1 (Q9H4M9).
偶联物
形式
Liquid
浓度
1.28 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2911955
产品详细信息
Positive test controls include: HeLa, THP-1, U-251MG, Mouse liver, Mouse brain, Mouse spleen, Rat lung, Rat testis, Rat kidney. The target is usually found in the following locations: Cell membrane, Cell projection, Cytoplasmic side, Early endosome membrane, Peripheral membrane protein, Recycling endosome membrane, cilium membrane.
Immunogen sequence: MFSWVSKDAR RKKEPELFQT VAEGLRQLYA QKLLPLEEHY RFHEFHSPAL EDADFDNKPM VLLVGQYSTG KTTFIRHLIE QDFPGMRIGP EPTTDSFIAV
靶标信息
EHD1 acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. EHD1 also plays a role in myoblast fusion and is recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth. Further, EHD1 belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. The EHD1 protein is thought to play a role in the endocytosis of IGF1 receptors. Alternatively spliced transcript variants have been found for the EHD1 gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: EH domain-containing protein 1; EH-domain containing 1; hPAST1; mPAST1; PAST homolog 1; Testilin
基因别名: AA409636; CDABP0131; EHD1; H-PAST; HPAST1; PAST; PAST1; RGD1306960; RME-1
UniProt ID: (Human) Q9H4M9, (Mouse) Q9WVK4, (Rat) Q641Z6
Entrez Gene ID: (Human) 10938, (Mouse) 13660, (Rat) 293692
intracellular protein transport
endocytosis
blood coagulation
positive regulation of cholesterol storage
neuron projection development
endocytic recycling
low-density lipoprotein particle clearance
cilium assembly
cholesterol homeostasis
protein homooligomerization
protein localization to cilium
cell-cell adhesion
positive regulation of myoblast fusion
cellular response to nerve growth factor stimulus
positive regulation of endocytic recycling
endosomal transport
