Invitrogen

EHD2 Polyclonal Antibody

EHD2 Antibody in Immunocytochemistry (ICC/IF)

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EHD2 Antibody (PA5-80576) in ICC/IF

Immunofluorescence staining of EHD2 in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with EHD2 Polyclonal Antibody (Product # PA5-80576, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.

EHD2 Antibody in Immunoprecipitation (IP)

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产品信息

PA5-80576

应用

建议稀释比

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免疫印迹 (WB)

1:500-1:2,000

免疫细胞化学 (ICC/IF)

1:300-1:10,000

免疫沉淀 (IP)

1-4 µL/mg of lysate

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to the C-terminus of the Human EHD2

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2787875

产品详细信息

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Human EHD2.

靶标信息

This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway.

仅用于科研。不用于诊断过程。未经明确授权不得转售。