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EHMT1 Antibody (PA5-114733) in ICC/IF
Immunocytochemistry analysis of EHMT1 in A549 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114733). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
产品信息
PA5-114733
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human EHMT1(Accession Q9H9B1), corresponding to amino acid residues C842-L892.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2899369
产品详细信息
Antibody detects endogenous levels of total EHMT1.
靶标信息
EHMT1, also known as EUHMTASE1, is a histone methyltransferase. This protein methylates 'Lys-9' of histone H3 in vitro. H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. EHMT1 is Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in the G0/G1 transition of the cell cycle.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Eu-HMTase1; Euchromatic histone-lysine N-methyltransferase 1; G9a-like protein 1; GLP; H3-K9-HMTase 5; Histone H3-K9 methyltransferase 5; Histone-lysine N-methyltransferase EHMT1; histone-lysine N-methyltransferase, H3 lysine-9 specific 5; Lysine N-methyltransferase 1D
基因别名: 9230102N17Rik; bA188C12.1; D330003E03; EHMT1; Eu-HMTase1; EUHMTASE1; FP13812; GLP; GLP1; KIAA1876; KMT1D; mKIAA1876
UniProt ID: (Human) Q9H9B1, (Mouse) Q5DW34
Entrez Gene ID: (Human) 79813, (Mouse) 77683
p53 binding
protein binding
methyltransferase activity
zinc ion binding
protein-lysine N-methyltransferase activity
histone-lysine N-methyltransferase activity
histone methyltransferase activity (H3-K9 specific)
histone methyltransferase activity (H3-K27 specific)
C2H2 zinc finger domain binding
rRNA (adenine-N6,N6-)-dimethyltransferase activity
protein C-terminal carboxyl O-methyltransferase activity
tRNA (guanine-N2-)-methyltransferase activity
C-methyltransferase activity
N-methyltransferase activity
O-methyltransferase activity
S-methyltransferase activity
RNA methyltransferase activity
mRNA methyltransferase activity
tRNA methyltransferase activity
protein methyltransferase activity
2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity
rRNA methyltransferase activity
rRNA (uridine-2'-O-)-methyltransferase activity
rRNA (adenine-N6-)-methyltransferase activity
DNA-methyltransferase activity
tRNA (guanine-N1-)-methyltransferase activity
rRNA (cytosine-C5-)-methyltransferase activity
selenocysteine methyltransferase activity
arginine N-methyltransferase activity
protein-arginine N-methyltransferase activity
lysine N-methyltransferase activity
tRNA (uracil) methyltransferase activity
tRNA (guanine) methyltransferase activity
tRNA (adenine) methyltransferase activity
tRNA (cytosine) methyltransferase activity
tRNA (cytosine-5-)-methyltransferase activity
tRNA (adenine-N1-)-methyltransferase activity
rRNA (adenine) methyltransferase activity
rRNA (cytosine) methyltransferase activity
rRNA (guanine) methyltransferase activity
rRNA (uridine) methyltransferase activity
transferase activity
protein C-terminal leucine carboxyl O-methyltransferase activity
1-phenanthrol methyltransferase activity
protein-arginine N5-methyltransferase activity
methylarsonite methyltransferase activity
dimethylarsinite methyltransferase activity
4,5-dihydroxybenzo(a)pyrene methyltransferase activity
1-hydroxypyrene methyltransferase activity
1-hydroxy-6-methoxypyrene methyltransferase activity
demethylmenaquinone methyltransferase activity
cobalt-precorrin-6B C5-methyltransferase activity
cobalt-precorrin-7 C15-methyltransferase activity
cobalt-precorrin-5B C1-methyltransferase activity
cobalt-precorrin-3 C17-methyltransferase activity
dimethylamine methyltransferase activity
hydroxyneurosporene-O-methyltransferase activity
tRNA (adenine-57, 58-N(1)-) methyltransferase activity
methylamine-specific methylcobalamin:coenzyme M methyltransferase activity
trimethylamine methyltransferase activity
methanol-specific methylcobalamin:coenzyme M methyltransferase activity
monomethylamine methyltransferase activity
metal ion binding
P-methyltransferase activity
Se-methyltransferase activity
2-phytyl-1,4-naphthoquinone methyltransferase activity
tRNA (uracil-2'-O-)-methyltransferase activity
tRNA (cytosine-2'-O-)-methyltransferase activity
phosphomethylethanolamine N-methyltransferase activity
tRNA (cytosine-3-)-methyltransferase activity
rRNA (cytosine-2'-O-)-methyltransferase activity
rRNA (cytosine-N4-)-methyltransferase activity
trihydroxyferuloyl spermidine O-methyltransferase activity
negative regulation of transcription from RNA polymerase II promoter
DNA methylation
embryo development
chromatin modification
histone methylation
peptidyl-lysine monomethylation
peptidyl-lysine dimethylation
negative regulation of transcription, DNA-templated
histone H3-K9 methylation
response to fungicide
histone H3-K27 methylation
regulation of signal transduction by p53 class mediator
methylation
histone lysine methylation
