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EIF2A Antibody (600-101-ML7) in ICC/IF
Immunofluorescence of Goat Anti-EIF2A Antibody. Cells: HeLa Cells. Fixative: 4% PFA. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-EIF2A at 5µg/mL overnight at 2-8°C. Secondary Antibody: Donkey Anti-Goat IgG DyLight™488 (p/n 605-741-125) at 5µg/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A) DAPI. (B) Anti-EIF2A and DyLight™488. (C) merge of A+B. (D). Secondary only. Localization: cytosol, extracellular >> mitochondria.
产品信息
600-101-ML7
产品规格
种属反应
Human,
Mouse
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Anti-EIF2A antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-terminal portion of human EIF2A conjugated to Keyhole Limpet Hemocyanin (KLH).
偶联物
形式
Liquid
浓度
1.08 mg/mL
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against human EIF2A. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 86.7% homology with the immunizing sequence to mouse.
靶标信息
EIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 65 kDa eukaryotic translation initiation factor 2A; eIF-2A; Eukaryotic translation initiation factor 2A; eukaryotic translation initiation factor 2A, 65kDa
基因别名: CDA02; D030048D22; D3Ertd194e; EIF-2A; EIF2A; MST089; MSTP004; MSTP089
UniProt ID: (Human) Q9BY44, (Mouse) Q8BJW6
Entrez Gene ID: (Human) 83939, (Mouse) 229317
