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EIF2S1 Antibody (MA1-079) in ICC/IF
Immunofluorescent analysis of eIF2S1 (green) showing positive staining in the cytoplasm of COS7 cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with an eIF2S1 monoclonal antibody (Product # MA1-079) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyL... View More
产品信息
MA1-079
应用
建议稀释比
已发表文章
产品规格
已发表种属
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
5A5
抗原
Full length human EIF2S1 produced in HEK293T cells.
偶联物
形式
Liquid
浓度
1 mg/mL
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2536736
产品详细信息
MA1-079 has been successfully used in Western blot, immunohistochemistry, immunocytochemisty and immunofluorescent applications and reacts with human, monkey, and rat samples.
靶标信息
EIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner to form the 43S pre-initiation complex. The polypeptide can be phosphorylated by related protein kinases activated in response to stress. Phosphorylated EIF2A inhibits EIF2B activity and prevents guanine nucleotide exchange.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
