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ELP3 Antibody (MA5-49241) in WB
Western blot analysis of KAT9/ELP3 using KAT9/ELP3 antibody (Product # MA5-49241). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human K562 whole cell lysates; Lane 2: human U20S whole cell lysates; Lane 3: human HepG2 whole cell lysates; Lane 4: human U87 whole cell lysates; Lane 5: rat lung tissue lysates; Lane 6: rat PC-12 whole cell lysates; Lane 7: mouse lung tissue lysates;... View More
Product Details
MA5-49241
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
8B4
Immunogen
A synthetic peptide corresponding to a sequence of human KAT9/ELP3 (RDPTKFQHQGFGMLLMEEAERIAREEH).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_3074899
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human K562 whole cell, human U20S whole cell, human HepG2 whole cell, human U87 whole cell, rat lung tissue, rat PC-12 whole cell, mouse lung tissue, mouse NIH/3T3 whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Elongator complex protein 3 (ELP3) is a catalytic histone acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4. It may also have a methyltransferase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EC 2.3.1.48; elongation protein 3 homolog; Elongator complex protein 3; h KAT9; hELP3; rCG52086-like; tRNA uridine(34) acetyltransferase
Gene Aliases: 2610507P14Rik; ELP3; KAT9
UniProt ID: (Human) Q9H9T3, (Mouse) Q9CZX0
Entrez Gene ID: (Human) 55140, (Mouse) 74195, (Rat) 100362548
RNA polymerase II core binding
protein binding
phosphorylase kinase regulator activity
H3 histone acetyltransferase activity
H4 histone acetyltransferase activity
metal ion binding
iron-sulfur cluster binding
catalytic activity
histone acetyltransferase activity
N-acetyltransferase activity
transferase activity
transferase activity, transferring acyl groups
DNA binding
neuron migration
tRNA wobble uridine modification
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
nervous system development
central nervous system development
histone acetylation
positive regulation of cell migration
histone H3 acetylation
histone H4 acetylation
regulation of protein kinase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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