Invitrogen

ENO1 Polyclonal Antibody

ENO1 Antibody in Immunocytochemistry (ICC/IF)

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ENO1 Antibody (PA5-102334) in ICC/IF

Immunofluorescent analysis of ENO1 in HEPG2 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% saponin, blocked with 10% serum (45 min at 25°C) incubated with ENO1 polyclonal antibody (Product # PA5-102334) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) at a dilution of 1:600.

ENO1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-102334

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:400

产品规格

种属反应

Human, Mouse, Non-human primate, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human ENO1(Accession P06733), corresponding to amino acid residues S370-K420.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2851742

产品详细信息

Antibody detects endogenous levels of total ENO1.

靶标信息

Enolase (2-phosphogly-cerate hydrolyase or phosphopyruvate hydrates) is a glycolytic enzyme that catalyzes the dehydration and conversion of 2-phosphoglycerate to phosphoenolpyruvate. It comprises three distinct subunits, alpha, beta and gamma. Non Neuronal Enolase(ENO1) is an isoform of mammalian enolase and is composed of 2 alpha subunits. The gene for ENO1 also encodes a shorter monomeric structural lens protein, tau-crystallin. Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.Used as a diagnostic marker for many tumors and, in the heterodimeric form, alpha/gamma, as a marker for hypoxic brain injury after cardiac arrest. Also marker for endometriosis. Antibodies against alpha-enolase are present in sera from patients with cancer-associated retinopathy syndrome (CAR), a progressive blinding disease which occurs in the presence of systemic tumor growth, primarily small-cell carcinoma of the lung and other malignancies. Is identified as an autoantigen in Hashimoto encephalopathy (HE) a rare autoimmune disease associated with Hashimoto thyroiditis (HT). HT is a disorder in which destructive processes overcome the potential capacity of thyroid replacement leading to hypothyroidism.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 2-phospho-D-glycerate hydro-lyase; 2-phospho-D-glycerate hydrolase; alpha enolase; alpha enolase like 1; Alpha-enolase; c-Myc promoter binding protein; C-myc promoter-binding protein; enoa; Enolase 1; enolase 1, (alpha); enolase 1, alpha non-neuron; enolase-alpha; epididymis secretory protein Li 17; MBP-1; MPB-1; MYC promoter-binding protein 1; NNE; Non-neural enolase; Phosphopyruvate hydratase; Plasminogen-binding protein; tau-crystallin

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基因别名: 0610008I15; AL022784; Eno-1; ENO1; ENO1L1; HEL-S-17; MBP-1; MBPB1; MPB1; NNE; PPH

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UniProt ID: (Human) P06733, (Mouse) P17182, (Rat) P04764

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Entrez Gene ID: (Human) 2023, (Mouse) 13806, (Rat) 24333

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